Table 2 Recommended specifications for Optical Microscopy measurements on heritage fibres

1

Manufacturer and instrument model

For documenting the quality of the instrument

2

Light source

What light source has been used with what settings?

3

Oculars

4

Objective

Manufacturer, Lateral Magnification, Numerical Aperture, Immersion Medium, Flat-Field Correction, Aberration Correction, Specialized Optical Properties, requested Tube Length, requested Coverslip Thickness, Working Distance

5

Condensor

Manufacturer, Magnification, Numerical Aperture

6

Köhler illumination

Has the instrument been adjusted for Köhler illumination after each objective change?

7

Camera, its mount and the software

What camera has been used and what were the settings?

8

Mounting medium

Type and value of refractive index

9

Cover slip: Thickness and material

Each objective Aperture tolerates a specific coverslip thickness and its deviation of Cover glass Thickness; the material influences the refraction of light in the light path

10

Operation mode

E.g. Bright Field

Polarized Light Microscopy

Phase Contrast