Analyses of the brown stain on the Parthenon Centaur head in Denmark

Heritage Science

Table 2 Proteins identified in samples during the last database search (against the refined multi-organism database)

UniprotID	Protein name (origin)	Number of identified peptides	Negative (preblank)	Negative	P1 (preblank)	P1	P2 (preblank)	P2	P3 (preblank)	P3	P4 (preblank)	P4	P5 (preblank)	P5
P02768	Albumin (primate)	2	×	×	×	✓	×	×	×	×	×	✓	×	×
P0DUB6	Alpha-amylase (mammalian)	3	×	×	×	×	×	×	×	×	×	✓	×	✓
P00690	Alpha-amylase (mammalian)	3	×	×	×	×	×	×	×	×	×	✓	×	✓
P28814	Barwin (cereal plants)	2	×	×	×	∆	×	∆	✓	∆	∆	∆	∆	✓
Q5R1M5	Chymotrypsin-like elastase 1 (mammalian)	2	×	×	×	∆	∆	×	×	∆	×	×	∆	✓
Q9UNI1	Chymotrypsin-like elastase 1 (mammalian)	5	×	×	×	∆	∆	∆	×	∆	×	×	∆	✓
P08217	Chymotrypsin-like elastase 2 (mammalian)	2	∆	×	×	×	×	×	×	×	×	×	×	✓
Q91X79	Chymotrypsin-like elastase 1 (mammalian)	4	×	×	×	∆	∆	∆	×	∆	×	×	∆	✓
P00773	Chymotrypsin-like elastase 1 (mammalian)	3	×	×	×	∆	∆	×	×	∆	×	×	∆	✓
P02453	Collagen alpha-1 (vertebrate)	7	×	×	×	✓	×	✓	×	∆	∆	✓	∆	✓
P02465	Collagen alpha-2 (vertebrate)	5	×	×	×	✓	×	✓	∆	∆	∆	✓	∆	✓
C0HJP0	Collagen alpha-2 (vertebrate)	3	×	×	×	×	×	✓	∆	×	∆	✓	×	✓
Q4A3R3	Hensin (mammalian)	2	∆	×	×	×	×	×	×	×	×	∆	×	✓
P02845	Vitellogenin-2 (avian)	2	×	×	×	×	∆	×	∆	∆	×	✓	×	∆

Symbols: × – not observed; ∆ – observed, by identification transfer; ✓– observed and identified. Identification transfer is a technique used in analysis of the related samples, when a peptide identified in one sample is transferred to another sample using its retention time and mass; pre-blank stands for the 0.1% FA injection immediately before the sample analysis (contamination control)