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Table 2 HPLC gradients

From: Colouring of Pacific barkcloths: identification of the brown, red and yellow colourants used in the decoration of historic Pacific barkcloths

Time (min)

Water (%)

Acetonitrile (%)

5% H3PO4 (%)

HPLC gradient 1

0

68

30

2

25

0

98

2

30

0

98

2

30.1

68

98

2

35

68

98

2

HPLC gradient 2

0

85

10

5

25

0

95

5

25.1

85

10

5

30

85

10

5