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Table 4 Chromophores of Iranian madder detected by RP-HPLC–DAD at 255 nm

From: Twentieth century Iranian carpets: investigation of red dye molecules and study of traditional madder dyeing techniques

  Name % RT Area_c
1 Ø 5.35 1.046 5.54
2 Ø 4.07 2.036 4.21
3 Unknown spectrum with max absorbance peaks 213–254–285–357 1.18 9.512 1.22
4 Unknown spectrum with max absorbance peaks 213–256–282–352 1.55 9.995 1.6
5 ~ Ruberythric acid (= very similar to rubetythric acid) 0.40 10.305 0.41
6 Ruberythric acid 1.80 11.732 1.86
7 Unknown spectrum with max absorbance peaks 251–278–344 0.47 13.012 0.49
8 Unknown spectrum with max absorbance peaks 219–258–272–339–410 0.65 15.211 0.67
9 Xanthopurpurin 0.70 15.988 0.72
10 Anthragallol 0.56 17.123 0.58
11 Pseudopurpurin + munjistin 11.28 17.772 11.67
12 Alizarin 39.54 18.873 40.92
13 Unknown spectrum with max absorbance peaks 223–252–284–436 0.87 19.905 0.9
14 Purpurin 1.22 22.46 1.26
15 ~ Rubiadin 0.68 25.291 0.7
16 Nordamnacanthal 29.36 26.84 30.38
17 Unknown spectrum with max absorbance peaks 214–245–270–280–391 0.19 28.756 0.2
18 ~ Xanthopurpurin 0.14 29.332 0.15
  1. Area_c Sum of peak areas (only identified peaks), RT retention time, Relative ratio (%) individual peak area divided by sum of peak areas multiplied by 100