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Heritage Science
Fig. 2 | Heritage Science

Fig. 2

From: Animal species identification utilising DNAs extracted from traditionally manufactured gelatin (Wanikawa)

Fig. 2Fig. 2

Species identification through DNA barcoding: a gelatin #6 (cattle) with PrimeSTAR; b gelatin #6 with MightyAmp DNA Polymerase, gradient PCR; c gelatin #7 (sheep) with MightyAmp DNA Polymerase, gradient PCR; d gelatin #3 (rabbit) with MightyAmp DNA Polymerase, gradient PCR; e gelatin #9 (fish) with MightyAmp DNA Polymerase, gradient PCR. In a, animal COI was originally set to be positive control. Upon employing MightyAmp DNA Polymerase, specific amplification was observed (c, d), possibly suggesting that wanikawa contains inhibitory components for PCR, such as humic acids. From the agarose electrophoresis, it is clear that gelatin #6 is made from cattle (a, b), gelatin #7 from cattle and sheep (c), whereas gelatin # 3 from rabbit (b). In contrast, no specific amplification was seen in gelatin #9 (e)

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