Table 1 The major histological changes observed at the different stages of skin transformation
Histological staining methods | HES | Green Trichrome | Orcein | Picro-Sirius red | Wilder | Oil Red O |
|---|---|---|---|---|---|---|
Histological specificity | Elastin | Type I Collagen | Type III Collagen | Lipids | ||
Sample #1: fresh skin | Classical topography | Classical topography | More Present at the limit dermis-hypodermis and just around hair follicles | Present everywhere Highest density just around hair follicles | Anchored in the basement membranes | At the apex of epidermis and in the sebaceous glands |
Sample #2: skin after hair removal (1.Watery lime bath; 2. Milky lime bath) | Loss of epidermis, hair follicles without hairs Evidence for expanded network of collagen, Persistent nuclei | Like HES | Persistent in the described zones | Evidence of expanded network of collagen | Basement membranes less emphasized | Persistent in sebaceous glands |
Sample 3: skin after 3 lime baths | Collagen gelled in a kind of pulp | Like HES | Present in traces | Collagen gelified in pulp | Present in traces | Persistent in sebaceous glands |
Sample #4: parchment | Layers in gelled collagen Horizontalisation of the glands | Like HES | Present in parallel traces | Quite uniform | Present in parallel traces or artefact? | Persistent in sebaceous glands |