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Heritage Science

Table 2 The gradient elution program for HPLC analysis

From: Characterization of materials in the late 15th-century animal encyclopedia at an Ottoman Library and detection of deterioration products by HPLC–DAD

Time (min)

Flow rate (mL/min)

H2O- 0.1% TFA (v/v)

CH3CN-0.1% TFA (v/v)

0.0

0,5

95

5

1.0

0,5

95

5

20.0

0,5

70

30

25.0

0,5

40

60

28.0

0,5

40

60

33.0

0,5

5

95

35.0

0,5

5

95

40.0

0,5

95

5

45.0

0,5

95

5

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