- Research article
- Open Access
Convento di San Francesco a Folloni: the function of a Medieval Franciscan Friary seen through the burials
© Torino et al. 2015
- Received: 8 January 2015
- Accepted: 23 July 2015
- Published: 1 September 2015
The Franciscan Friary in Montella near Avellino in Southern Italy is of special interest because according to historical sources it was founded by St. Francis himself in AD 1221–1222. Human remains of several hundred individuals interred in the cloister walk have been unearthed during two excavation campaigns conducted in 2007–2008 and 2010. The environs of the friary have remained rural since the foundation preventing much modern contamination. The state of preservation of the skeletons is fair to good making a suite of analyses worthwhile.
The skeletons have been examined anthropologically and tissue samples have been subjected to radiocarbon dating, stable isotope measurements and trace element analyses by Inductively Coupled Plasma Mass Spectrometry and Cold Vapour Atomic Absorption Spectrometry.
The radiocarbon dates are consistent with the historical sources and show that the cemetery in the cloister walk has been in uninterrupted use from the foundation of the friary in AD 1221–1222 and until the cemetery went out of use in AD 1524. The anthropological investigations show that the individuals interred at the friary would have been shorter than other Italians from the same time, and it seems that tuberculosis was more prevalent than leprosy. Isotopic measurements show a mixed agricultural and pastoral diet and none of the individuals were consuming marine protein. Based on the trace element analysis it seems that the people resided mainly at two distinct geographical areas, one of which was Montella. One individual stands out from the rest, because he was born and raised at some third geographical location distinct from Montella and because he sports the second oldest radiocarbon date of AD 1050–1249 (two sigma calibrated range). This date is consistent with the first generation of the founders of the friary—perhaps one of St. Francis’ fellow travellers from Assisi.
- Franciscan friary
- Trace elements
- Stable isotopes
The purpose of this paper is to investigate the relationship between the friary and the surrounding society and in this way gain knowledge about the growth of a monastic settlement over 300 years. The monastic institutions formed the background for the social and political order in Europe throughout the medieval period. In order to fulfil this purpose, three different types of analyses have been carried out: an archaeological analysis of the building and burial history of the site based on observations from two excavations; an osteological analysis of the skeletons interred in the friary; and chemical analyses including 14C dating, stable isotopes and trace elements of individual skeletons to describe the life and migration history of the people interred in the friary.
The cloister walk was the only compartment that was left on its original footprint from the old complex. The church was rotated 90° around the altar which today is still at the same position as it was in the 13th Century church, and it is still elevated ca. 180 cm over the church floor. The front entrance of the friary was replaced by the installation with two rows of columns (antichiostro). The southern corridor of the new cloister walk is only in contact with the old cloister walk over 2.5 m. This small overlap can be seen in the drawing of the layout of the buildings erected in 1740–1741 made immediately before the repair work took place ([4, 5]; Fig. 2).
In the fall of 2007 a small exploratory excavation was carried out aimed at assessing the archaeological potential of the area. The main excavation lasted for a year and covered most of the cloister walk with a total area of approximately 160 m2. Another excavation followed in August 2010 where a field of the southern corridor of the cloister walk (an area of 18.5 × 2.5 m) was excavated. The modes of investigation of the two excavations were different. The 2007–2008 excavation was scheduled as an educational excavation with 12 archaeology students lead by M. Torino. The 2010 excavation was led by S. Schiavone and was conducted for the local Archaeological Service in order to give way for repaving work.
The first excavation revealed 23 graves in two corridors, A and B (see Fig. 2), of the four corridors of the cloister walk. Seen from an archaeological point of view the graves seem to belong to three different time periods. One of the oldest burials (T-4 USD230) was oriented east–west and situated in the cloister garden. This grave represents the earliest element of the development of the cemetery. Five other tombs from this period have been identified (T-20 USD295, T-18 USD350, T-1 USD-210, T-14 USD274, and T-13 USD-265).
Three burials, including a double grave, were destroyed by the re-construction of the friary, with the building of the nave of the church on the south side. The building activity at this place probably took place by the end of the 13th Century and the first quarter of the 14th. The remaining burials respect the orientation of the two orthogonal arms A and B, and can be referred to the last period of the funerary activity lasting apparently without interruption until 1524. Archival documents from 1524 indicate the existence of a wrought of mortar which was identified in its full extent in the excavation of the north corridor.
The 2010 excavation revealed a greater number of skeletons, probably due to the more prominent position near the entrance of the church; the area can be identified as the paradisus. There are 38 primary burials and ca. 12 burials which have been cut by later activity in the soil. The observations of the second excavation are consistent with the burials unearthed in the first excavation and can also be interpreted as belonging to three periods. Nine graves can be assigned to the early period, including 3 from a masonic tomb which includes a double grave where the bodies were positioned on top of one another (USD 397 and 408).
In the late period of use two separate areas of graves were found in rectangular shaped areas (3.86 × 1.65 and 4.18 × 1.40 m, respectively), each with ca. 11 graves and two collective tombs buried in two trenches (3.92 × 1.43 m) separated by a strip of sterile soil between them. The burial pattern suggests that these graves could have been almost contemporary.
Osteologically, the Montella skeletons were examined in two rounds. The first round was aimed at collecting general data on size, sex, age and diseases. These data were collected to facilitate a reconstruction of the characteristics of the population at the site while it was still alive. The second round of examinations was aimed at collecting in-depth information about the life (and death) of selected individuals in order to get a better understanding of single individuals.
Sex was primarily estimated from traditional methods which include differences on the pelvis and on the skull . Furthermore measurements of the femoral head , the humeral head and the humeral epicondyle  were used to assess the sex. It must be stressed that osteologists assess sex, viz. the biological aspect of the difference between men and women whereas archaeologists tend to assess gender, the cultural aspect of the difference between men and women. Primarily, sex was recorded on a 1–7 scale. The meaning of these scores are (1) definitely male (M); (2) probably male (M?); (3) possibly male (?M); (4) undetermined (?—children end up in this category); (5) possibly female (?F); (6) probably female (F?); and (7) definitely female (F). In the further analyses, codes 1 and 2 are treated as males and codes 6 and 7 as females.
Age at death
Age at death was estimated using the so called ‘experience-based method’ . The method is based on primarily so far undescribed anatomical traits. The skeletal characters traditionally used for age estimation like closure of the cranial sutures, changes on the pubic symphysis and the auricular surface, and transformation of the sternal end of the 4th rib provide much less accurate age information and the statistical approach used to generate age standards from these characters are, with the exception of Transition Analysis , seriously biased . The experience–based method is based on data from all parts of the skeleton, as it utilizes virtually every bone in the body—most notably the vertebrae, proximal femur and the ribs.
The femoral length was measured on all skeletons with fused epiphyses. This was the only anthropometric measurement included in the general description of the population. It would, of course, be valuable to have information about stature in the once living population but due to burial custom and conditions of preservation it was not possible to measure the length of the buried bodies in accord with the method described by Boldsen .
Skeletal trauma are coded independently of them being perimortal (fresh) or healed. Analyses of trauma were not a main purpose of the research into the Montella skeletons. So in the general examination traumatic lesions were only recorded to one of the bodily regions: Head, arms, legs and the rest.
The four largest joints in the body—shoulder, hip, knee and ankle—were scored for changes in the joint. Minor changes occur in virtually everybody beyond the age of 30 and they were probably not associated with any discomfort. As a consequence of this only major joint changes were recorded. Joints with either eburnation on the surface of the joint or extensive lipping/ostephythosis along the rim of the joint were classified as positive for joint degeneration. Extensive lipping or ostephythosis is defined as sharp bony outgrowth along the edge of the joint being at least one cm long and extending at least one mm over the surface of the joint.
Tuberculosis can leave its characteristic marks virtually all over the body. In the present study, however, such lesions were only recorded in the spine. The nutrient foramina in the vertebrae can enlarge to 3 mm with a funnel shaped opening due to tuberculosis. Individuals were considered unaffected if they had normal sized and shaped nutrient foramina. Where the cranial and/or the caudal surfaces of the vertebral bodies show invasive porous destruction and depressions this is considered to indicate tuberculosis; this is also the case for Pott’s disease, and wedge shaped vertebras that can be seen as advanced cases of the destruction just described . The spinal lesions are recorded separately for the thoracic and the lumbar region of the spine.
For the registration for leprosy seven anatomical traits are examined. These traits are: (1) the edge of the nasal aperture that can be ‘normal’ sharp or rounded and affected. (2) The anterior nasal spine can be pointed (unaffected) or degenerated or rounded (affected). (3) The alveolar process of the pre-maxilla can be normal and unaffected or can be retreated (affected). (4) The palate can appear with no or little porosity (unaffected) or with densely pitted or perforated palate (affected). The fibula can appear with a smooth surface (unaffected) or fibula with subperiostal exostoses (affected) (5) or a swollen fibula (6). (7) The fifth metatarsal bone can appear with a normal surface (unaffected) or with periostitis or exostoses or shortened and deformed .
The statistical analyses of the anthropological observations were carried out using SPSS (v. 21.0). For the most parts standard statistical methods like cross-table analysis, un-paired t test and Kaplan–Meier survival analysis using the Mantel-Cox log-rank test applied to test for effects of covariates like sex or disease status.
The diagnostic and epidemiological analysis of leprosy is statistically far more developed than the analysis of any other disease in the past. Here we have used the λ-method first for estimating the sample frequency of leprosy. This statistic could be named the point prevalence of leprosy at death in the population buried at the Montella friary. Once this frequency is estimated, individual skeleton disease status can be assessed—of course as probability statements. This approach has been developed and described previously [15–17].
Sampling of the bone for trace element analyses
The sampling of the bones took place at the premises of the friary 2 years after the excavations. Great care was taken to avoid contamination during the field sampling. Laboratory coats, plastic gloves, hairnets and facemasks were used throughout the sampling process. A large sheet of white paper was covered by two layers of Al-foil on top of which the bone was placed. The drill used was a conical drill bit mounted on a Dremel MultiPro® electric drill. The drill bit and the other utensils used for handling the bone samples were rinsed in MilliQ® water and heated to dryness in an ethanol flame before drilling in order to decontaminate the utensils of any Hg possibly present. A small area of bone surface was drilled off down to ca. 1 mm and discarded together with the gloves and the top layer of Al-foil. The utensils were cleaned again in MilliQ water and heated in the ethanol flame. A new sheet of Al-foil was added and new gloves were used. The bone sample was drilled in the decontaminated area without touching the remaining bone surface surrounding the decontaminated area. Approximately 50 mg granulated bone was transferred to a pre-cleaned glass vial and sealed.
The trace elements were analysed by ICP-MS and CV-AAS at the CHART laboratory (Cultural Heritage and Archaeometric Research Team at SDU). The sample weight used for ICP-MS analysis was approximately 20 mg . The bone samples were handled by a stainless steel spoon, which was rinsed in MilliQ water and heated in an ethanol flame between each sample. After weighing the sample was transferred to a 50 mL polypropylene centrifuge tube. To dissolve the sample, 2 mL of conc. 69% ICP-MS grade HNO3 and 1 mL of conc. 30% ICP-MS grade H2O2 were added to the tube. Subsequently the lid was loosely fitted on tube and the tube was placed on a shaking table for no less than 3 h. The amount of H2O2 consumed in the dissolution process depends on the amount of organic compounds present in the sample, which varied from sample to sample. To remove the surplus H2O2 and to dissolve any possibly remaining solids, 335 µL of conc. 37% ICP-MS grade HCl were added, after which the tube with the lid loosely fitted was placed on the shaking table overnight.
The samples were diluted quantitatively to 10 mL with MilliQ water and then filtered through a 0.45 µm PVDF Q-Max® RR Syringe filter into a 15 mL polypropylene centrifuge tube. Further quantitative dilution was done in the 15 mL tubes according to the concentrations of specific sample, for normal samples using 3 mL sample solution and 9 mL MilliQ water. This way the diluted sample acquired an acid concentration of approximately 1%, which is well suited for analysis. The remaining filtrate was stored in the sealed tubes in a refrigerator at 5°C for CV-AAS analysis for Hg.
The ICP-MS analyses were carried out using a Bruker ICP-MS 820 equipped with a frequency matching RF-generator and a Collision Reaction Interface (CRI), the latter operated with either helium or hydrogen. The samples were introduced into the system using a Bruker SPS3 autosampler and an OneFast flow injection inlet system. The basic parameters were as follows: radiofrequency power 1.40 kW; plasma gas flow rate 15.50 L min−1; auxiliary gas flow rate 1.65 L min−1; sheath gas flow rate 0.12 L min−1; nebulizer gas flow rate 1.00 L min−1. The following isotopes were measured without skimmer gas: Mg24, Al27, Ca44, Mn55, Zn66, Sr88, Ba137 and a combination of Pb isotopes (Pb206, Pb207 and Pb208). The CRI Reaction System was activated for Cu and Fe because of interferences with polyatomic species produced by a combination of isotopes from the argon plasma, reagents and the bone matrix. Fe56 was measured with hydrogen as skimmer gas and Cu63 with helium as skimmer gas. A mixture of Sc45, Y89 and Tb159 was used as internal standard and added continuously to all samples. The dwell time on each peak was between 5 and 20 ms. Five replicate analyses were made of each of the dissolved bone samples and each replicate consisted of 30 mass scans. ICP multi-element standard solution XXI for MS (Merck) were prepared in 1% HNO3 at 6 different concentrations (0.1, 1, 10, 20, 100 and 200 μg L−1), but for each element only 3 of the standards were selected to fit the appropriate concentration range in the samples. For the main element Ca three standards (Fluka TraceCert® ICP Standard) of concentrations 10, 20 and 25 wt% were used. Elements showing higher than expected concentrations had their count rates attenuated automatically in the detector. A sample of water and a blank sample consisting of 1% HNO3 were run before the standard blank to ensure that no pollution was lingering in the system at start up. A blank sample was analysed between each bone sample in order to rinse the system and avoid any memory effects. Each working day an in-house standard sample made of a homogenized medieval bone was analysed along with the samples in order to monitor the overall performance. Together with the samples was run an international standard sample, NIST SRM-1486, a modern bone sample. For these modern samples more H2O2 had to be added.
Mercury was analysed by CV-AAS. After weighing, ca. 20 mg of sample was transferred to a centrifuge tube. The bone powder was dissolved in 2 mL conc. 69% ICP-MS grade HNO3 and 1 mL conc. 30% ICP-MS grade H2O2 . Afterwards the tubes were placed on a shaking table for 3–4 h; following which 0.335 mL concentrated 37% ICP-MS grade HCl was added in order to remove any superfluous H2O2. The samples were left overnight on the shaking table with the caps loosely fitted. The solution was diluted with 16 mL of MilliQ water. 1 mL of 5 ppm KMnO4 was added in order to make sure that the Hg was kept in the oxidized state. The samples were placed on the shaking table for at least 2 h to allow the reactions to run to completion. Five standard solutions in the concentration range 25–200 ppb were prepared using a PerkingElmerPure mercury nitrate standard solution in diluted HNO3 (1.5%). The Hg was measured by cold vapour atomic absorption spectroscopy on a Flow Injection Mercury System 400 manufactured by PerkinElmer. The Hg was released as vapour in the FIMS-400 by adding NaBH4 (0.2%) in 0.05% NaOH. The analyses were then run in triplicate in an alternating sequence with a blank sample between each of the bone samples. A solution of the in-house medieval bone standard was measured each day in order to check performance.
Fifty samples were radiocarbon dated at the CIRCE laboratory (Centre for Isotopic Research for Cultural and Environmental heritage). The commonly analysed fraction for bone radiocarbon dating is the collagen. In order to remove any contaminants introduced after death and to extract the collagen fraction, all analysed bone samples were treated using a protocol known as Gelatinization. This process is effective in removing contaminants on samples younger than 20,000 years [20–22].
The bone samples were crushed and treated with the AAA protocol, a series of acid and basic attacks, in order to remove carbonates and humic acids and to extract the collagen. Then the GEL fraction was obtained by heating the collagen to 70°C in a solution with pH 3 for 20 h . Finally the gel was freeze-dried and used for the successive combustion and graphitization processes [24, 25]. The obtained graphite was measured for the 14C/12C isotopic ratio using a NEC 3 MV accelerator mass spectrometer . The measurements were expressed as 14C-ages calculated according to Stuiver and Polach  and calibrated by using the OxCal v4.2.3 program  and the IntCal13 calibration curve .
Paleo diet analyses
Stable isotopes analysis of human collagen has proven to be a reliable method for reconstructing the diet of ancient populations [30–32]. Isotope composition, i.e. stable isotope ratios of carbon (C) and nitrogen (N), expressed in term of delta notation (δ13C and δ15N), in the body tissues reflect that of the consumed food. The principles, allowing the reconstruction of human paleo diet from stable isotope analysis are well known: bone collagen, with its turnover rate of around 3% per year (for a typical male adult), expresses a representative record of an individual’s diet, albeit between diet and bone collagen there is an offset of 5‰ in δ13C and 3‰ in δ15N. Isotopic signatures at the origin of a trophic chain characterize the chain itself (for example, marine or terrestrial). The increase of δ13C in human diet arises mainly as a result of the consumption of two types of 13C-enriched foods. Firstly, marine foods are characterized by δ13C values ~7‰ higher than terrestrial foods. Secondly, different photosynthetic pathways fractionate carbon isotopes differentially; therefore C4 plants have δ13C values of their tissues about 17‰ higher than the corresponding components of C3 plants. Using C isotopes alone these two 13C-enriched sources (C4 plants and marine foods) cannot be distinguished from one another, or reveal the consumption of animal derived protein. Therefore, δ15N in collagen is necessary auxiliary information. Much of the variation in δ15N largely arises as a result of the trophic level effect: carnivores have higher δ 15N values than herbivores, whom they feed on, and the greater is the number of trophic levels between a prey and its predator, the larger is the difference in their δ15N. The number of trophic levels in the marine and aquatic realms is greater than on land. Therefore the consumption of small fish is unlikely to produce high δ15N values because they are low trophic level feeders. Moreover, the highest δ 15N values in humans are observed where marine or aquatic foods are important in the diet. Humans that consume only terrestrial protein sources may have collagen δ 13C values of approximately −20‰, and δ15N values ranging from 5 to 12‰, while humans that consume marine food have collagen δ13C values close to −12‰ and δ15N values between 12 and 22‰. On the other hand, humans who consume a mixture of terrestrial animal and marine protein would have isotopic values somewhere between the above end points. This general schematization, useful to the understanding of the measurement results, is not universally valid; it could differ from site to site due to local contamination causing systematic variation of the whole food chain. Therefore, the site must be characterized by measuring faunal remains (e.g. sheep, cattle) for reference purposes since their role in the trophic levels is well known and constant.
The stable isotopes were measured at the SUN IRMS Laboratory at CIRCE (Centre for Isotopic Research for Cultural and Environmental heritage). As in the case of radiocarbon dating, the samples for the paleo diet analyses were processed to isolate the collagen. This was done using a modified procedure of the method described by Longin . The bone surface was abraded to remove any contaminants and the sample was pulverized. Each sample was placed in a polypropylene test tube and demineralised in a sequence of acid treatments with 0.6 M HCl at room temperature (20–25°C), interrupted by a 30-min alkaline treatment with 0.1 M NaOH. Several rinses with de-ionized water were carried out after each step, until neutrality was achieved, before drying the samples in an oven. Finally the gelatinization protocol  was applied. In order to test the quality of the collagen, C and N fractions of dry collagen were measured by an elemental analyser (CN Flash EA 1112, Thermo Scientific, Bremen) and expressed in % C and % N. Samples were retained for isotope analyses provided the extracted collagen achieved a yield higher than 1% and an atomic C:N ratio between 2.9 and 3.6 [33–35]. δ15N and δ13C were measured concurrently in continuous flow mode on a Thermo Scientific Delta Plus mass spectrometer.
Age at death, sex and mortality
It has been possible to estimate age at death of 321 individuals. Of these skeletons only 9.3% (30 individuals) were infants and young children up to the age of 5 years. The number of older children and adolescents (aged 6–19 years) was larger—78 skeletons corresponding to 24.3%. The remaining 213 skeletons (66.4%) were of adult individuals aged 20 years or more. The relatively low number of infants and young children probably reflects poor preservation of the more fragile skeletons of the young. The ratio of older children and adolescents to adults (78/213 = 0.366) corresponds to what can be expected from medieval mortality profiles at other cemeteries in Europe.
It was possible to estimate the sex of 217 skeletons aged 15 years or more. There were 134 males and 83 females. There were 16 skeletons in this age group for which it was not possible to estimate sex reliably. This overrepresentation of males (134 males against 83 females) is in accordance with a hypothesis that a substantial number of the interred individuals were indeed friars.
Descriptive statistics for femoral length of the skeletons from Montella and samples of Middle Italian medieval skeletons (data from Giannecchini and Moggi-Cecchi, 2008)
It was possible to find evidence for skeletal traumatic lesions in a total of 70 skeletons. Of these ten percent (7 individuals) had a traumatic skeletal lesion in one of the four body regions examined (head, arms, legs and torso). It appears that the legs were the least affected region of the body and the arms the most affected. Of the seven individuals with traumatic skeletal lesions, four had lesions in only one region, two had lesions in two regions and one skeleton showed traumatic lesions in three regions of the body. This last individual was attacked and killed by numerous blows with a sharp instrument. The frequency of trauma was virtually the same for men and women (p = 0.92) and individuals with and without traumatic skeletal lesions died at very similar ages (p = 0.27). The sample comes from a rather special cemetery: the individuals paid to be buried in the cloister walk of a friary, but they still had to be worthy of praise as devoted Catholics. It is therefore less likely they died as a consequence of violence or that they committed suicide. In this light the low level of skeletal trauma caused by violence is to be expected. The case of the USD 295 is an exception to the rule, as his unusual trauma proves.
In 48 skeletons at least one of the four major appendicular joints—shoulder, hip, knee and ankle—could be examined. Ten of these skeletons (21%) showed such changes in at least one joint. The affected joint was most frequently the knee (in 13% of the skeletons) and the least affected joint was the shoulder (4%). It appears that men and women were equally prone to developing joint degeneration (p = 0.44). Degenerative joint changes are age progressive. Once they have occurred they do not disappear again. Following this, it is not surprising that people who died with this condition were on average nearly 17 years older than those who died without them (p = 0.00021).
Leprosy and tuberculosis
It is commonly assumed that leprosy is a disease that people died with but not a disease that they died from. In the sense that leprosy rarely was or is the proximal cause of death, this is probably true. However, it is unlikely that people with leprosy did not experience elevated risks of dying compared with those who avoided contracting the disease. So, it is of interest to analyse the distribution of age at death among people with and without leprosy. As demonstrated above, there is a difference in the distribution of age at death for men and women and as there is only one female skeleton with leprosy the survival analysis could only meaningfully be carried out for males. In a figure illustrating the mortality profiles (estimated through Kaplan–Meier analysis) for men with and men without leprosy (figure not shown), and using the Mantel-Cox log rank test this difference was found to be statistically significant: χ2 = 4.53, df = 1, p = 0.033. It appears that men with leprosy on average died at higher ages than men without leprosy did. The mean difference in age at death in the two diagnostic groups is approximately 9 years. In a large pre-antibiotic data set  the median age at debut with leprosy was between 35 and 40 years. The difference between the mean ages at death in the two diagnosis cohorts can easily be accounted for by the selection effect of a late age at debut with leprosy. On the other hand, given the magnitude of the age at death difference selective mortality for leprosy must have been very slight.
Although the leprosy frequency in the Montella sample did not reach the level commonly seen in Southern Scandinavia , leprosy was by no means an unknown disease in Southern Italy in the Middle Ages.
Tuberculosis was diagnosed with more than one characteristic lesion in nine skeletons out of 33, which is ca. 27%. This frequency is in accord with findings from other European cemeteries, e.g. in Denmark: 22% in Viborg Skt. Morten , 35% out of 600 skeletons from Odense .
Results of radiocarbon dating: lab. no. are radiocarbon laboratory codes
14C age (BP) ± 1σ
Calibrated age (AD. 1σ)
Calibrated age (AD. 2σ)
709 ± 39
864 ± 26
656 ± 25
560 ± 15
584 ± 24
618 ± 17
781 ± 23
550 ± 29
517 ± 15
573 ± 38
403 ± 40
546 ± 18
687 ± 26
641 ± 28
504 ± 36
673 ± 23
577 ± 22
435 ± 23
642 ± 26
639 ± 33
813 ± 25
862 ± 66
586 ± 61
612 ± 41
686 ± 36
667 ± 32
647 ± 44
552 ± 28
614 ± 24
589 ± 43
526 ± 23
410 ± 38
802 ± 42
787 ± 41
694 ± 34
654 ± 29
747 ± 30
609 ± 31
1,061 ± 36
617 ± 25
670 ± 29
856 ± 30
826 ± 35
731 ± 38
554 ± 33
861 ± 41
590 ± 27
769 ± 74
925 ± 44
732 ± 34
Diet and stable isotopes
Results of stable isotope measurements on the bone collagen
The δ13C and δ15N average and standard deviation for the two subsample concerning primary and secondary graves has been calculated. No differences are found for the two subsamples as it is clear from the isotopic results: (1) primary graves (n = 41) δ13C = −19.7‰ (±0.8), δ15N = 6.9‰ (±0.9); (2) secondary graves (n = 7) δ13C = −19.4‰ (±0.2), δ15N = 8.3‰ (±1.2).
It is clear that none of the individuals were consuming marine protein, as all the δ13C are below −18.0‰ VPDB and the δ15N values are lower than would be expected for a marine based diet. This is not surprising considering the location is far from the sea.
It can also be ascertained from the δ15N values, which range from 5.3 to 10.1‰, that the diet did not include freshwater fish. This is perhaps more surprising as there is a creek less than 100 meters from the convent. Without isotopic measurements for fish/shellfish in lakes/rivers near Montella this is, however, harder to interpret. The absence of freshwater fish in the diet is fortuitous as it could otherwise give rise to a reservoir effect as has been reported in several places (see e.g. ). The radiocarbon dates can therefore in all likelihood be trusted for all of the dated individuals.
The results of the analyses by ICP-MS and CV-AAS are listed in Additional file 1: Table S4. Limits of quantization, LOQ, are calculated based on quality control runs performed over an at least 4 months period of regular use of the ICP-MS and the CV-AAS.
Tissue related differences in the abundances were seen for several of the elements. Zinc, Pb and Mg were more abundant in dentine than in bone; whereas Hg was more abundant in bone. There were no statistically significant differences between element concentrations or elemental ratios among the three different types of teeth (incisor, canine and wisdom tooth). In bone samples, it appears that Ba was more abundant in trabecular bone than in the two other types of bone sample. Lead was less abundant in compact bone than in the two other types of bone. More detailed interpretations follow below.
Bones in the human body undergo re-modelling via the formation of osteons [43–47]. After adolescence the re-modelling process continues throughout the remainder of life. The average turnover time is different for different tissue types. The cortical bone tissue generally exhibits an average turnover rate of ca. 2.5% per year and trabecular bone tissue has a faster turnover rate of ca. 10% per year [44, 48]. There is one exception, namely pars petrosa which contains the organs of the inner ear. This bone is fixated (hence the name) at around the age of 2 years. Teeth also undergo very little re-modelling once formed, and the root of the first incisor (+1) reflects the chemistry around the age of 9–10, the canine (+3) the age of 11–15, and the third molar (+8) 18–20 years of age .
Provenance, i.e. the geographical site of residence, can be revealed individually or by a cross plot of the elemental ratios Sr/Ca and Ba/Ca [18, 50]. Like the Sr-isotope ratios (see e.g. Price et al. ) the elemental ratios Sr/Ca and Ba/Ca are less controlled by metabolic processes and more by the trace elemental contents of water and food, the latter mainly locally grown vegetables which are also strongly dependent on the Sr and Ba content of the surface waters available. In an individual residing at the same geographical location throughout life it is therefore to be expected that all the bones exhibit more or less similar Sr/Ca- and Ba/Ca-ratios in the various bone elements, with the caveat that changes in metabolism may possibly introduce minor changes in the Sr and Ba uptake with age.
In many individuals the Hg concentration appears to have risen with age—it is lowest in pars petrosa, higher in compact bone and highest in trabecular bone. This could be due to an accumulative effect. Alternatively, the Hg concentration in the trabecular tissue could be interpreted to reflect the sum of the Hg in the bone tissue and the Hg originally present in the soft tissue, e.g. bone marrow, which is now decomposed and which will have left any Hg adhered to the surface of the lamellae of the trabecular bone. It has not been possible to mechanically decontaminate the trabecular bone tissue in the same thorough way it was done with the compact bone tissue samples.
Mercury was used for medical treatments already in the early medieval times [19, 52–54], but it got more and more popular during the late medieval times as a cure against syphilis. Mercury poisoning almost never leads to pathological bone lesions  and the use of Hg cannot therefore be diagnosed by looking at the bones. Normal non-exposed Hg-levels in compact bone tissue from medieval Denmark were reported by Rasmussen et al.  to be in the range 10–100 ng g−1; levels higher than these could indicate a treatment with an Hg-containing medicine at some point in the life of the individual.
There are other possible sources of Hg in a medieval society, for instance the use of cinnabar (HgS) as a colour pigment in manufacturing paintings, books and parchments, or the processes of gilding metallic objects. However, the data from the present study speaks in favour of the total absence of Hg in the monastic environment of Montella in terms of Hg-containing medicine, widespread use of cinnabar (HgS) in the scriptorium of the friary or other sources.
Contamination and diagenesis
It is evident that the material least exposed to groundwater contamination is the teeth and the compact bone tissue, while the pars petrosa and the trabecular bone tissue, which is much harder to decontaminate and much more exposed to contamination by percolating ground water and invading soil particles, are more exposed to diagenesis.
Calcium content of compact femoral bone tissue
The men interred in the cloister walk in Montella were on average 6–7 cm shorter than was normal in central Italy in the Middle Ages. Nine skeletons out of 33 (27%) showed signs of tuberculosis, while only five out of 44 (11%) individuals likely suffered from leprosy. Treponema diseases were not observed. As commonly seen in medieval populations male mean age at death was higher than female age at death (36.8 versus 33.4 years for individuals surviving to 15 years). In Montella this difference is statistically significant (p = 0.028).
Fifty individuals were radiocarbon dated. The distribution of the radiocarbon dates is consistent with a more or less continuous use of the cemetery from the foundation in AD 1221 and until 1524, in fine accordance with the historical sources. One individual most likely pre-dated the foundation of the friary in AD 1221–1222. The stable isotope measurements indicate the absence of both freshwater and marine reservoir effects, which leads to the conclusion that the radiocarbon dates can all be trusted to be accurate. The stable isotope measurements indicate a mixed agricultural/pastoral diet.
None of the investigated individuals had elevated Hg-concentrations, neither in the compact nor the trabecular tissue. There are therefore no indications of the use of Hg in the scriptorium as red ink (cinnabar) or as Hg-containing medication in the friary or in the village.
A unique feature for the individuals interred in this cemetery is that there is observed a correlation between age at death of the individual and the Ca-concentration of the compact tissue of the femur. It is not known what caused this condition, which is not seen in other cemeteries.
Based on the measured Sr/Ca and Ba/Ca ratios it is suggested that all the investigated individuals except USD-230 lived a substantial part of their lives either in Montella or one other location (“Site X”). USD-230 is the most outstanding of the individuals in terms of mobility. Based on the Sr/Ca and Ba/Ca elemental ratios it seems likely that only the individual USD-230 was born at “Site Y”, distinctly different in Sr and Ba inventory from Montella and “Site X”. USD-230 was radiocarbon dated to a two sigma calibrated range of AD 1050–1249, which is second-earliest individual dated at the cemetery. The date is consistent with the first generation of the founders of the convent—perhaps it was a fellow traveller of St. Francis of Assisi, or perhaps it was a burial that pre-dated the foundation of the friary.
SS lead the excavations. MT, JLB and PT conducted the osteological investigations. KLR, LS and LN made the sample preparations and conducted the trace element determinations. FT, IP, PR and CL made the radiocarbon dating and measured the stable isotopes. All authors read and approved the final manuscript.
The Friary of San Francesco a Folloni, Montella is thanked for permitting the excavations and the analyses. Agnello Stoia is thanked for continuing support from the start of this project. We are indebted to his never failing permissions, engagement and enthusiasm for the research conducted in the Montella Friary. Raffaele Coppola and Flavio Petroccione, Provincia di Avellino, are thanked for helping the project underway. Giovanni Coppola and Giancarlo Fatigati, University Suor Orsola Benincasa are thanked for continuous help and support during the project. Paolo D’Imporzano is thanked for helping with the literature studies.
Compliance with ethical guidelines
Competing interests The authors declare that they have no competing interests.
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