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Burial-induced deterioration in leather: a FTIR-ATR, DSC, TG/DTG, MHT and SEM study

Heritage Science volume 10, Article number: 7 (2022) Cite this article

Abstract

In this study we used an analytical approach based on complementary techniques that targets all structural levels of collagen in leather to investigate how vegetable-tanned leather deteriorates during soil burial tests. For the first time, a group of deterioration markers specific to molecular, fibrillar and fibrous structure of collagen in leather was associated with the deterioration of buried leather. The application of the second order derivative of FTIR-ATR spectra analysis allowed us to detect loosening of collagen–tannin matrix, de-tanning and gelatin formation based on the behaviour of collagen and tannin spectral components (intensity variation and shifts). Collagen denaturation observed by DSC analysis and its thermo-oxidative behaviour measured by TG/DTG analysis, as well as the altered morphology of collagen (namely melt-like fibres and distorted fibrillar ultrastructure) imaged by SEM confirmed the FTIR-ATR analyis results. These analytical outcomes enabled us to understand the effect of leather hardening/cementing through soil mineral penetration into its fibrous structure and thus correctly interprete the higher-than-expected shrinkage temperatures and intervals determinatd by MHT method. Thus, MHT method proved to be suitable for a quick evaluation method that can direcly support the first conservation decision after excavation. The combination of FTIR-ATR, DSC, TG/DTG and SEM can be particularly useful to provide insights on the deterioration mechanism of archaeological leather and support best decision on its long-term preservation.

Introduction

Materials, whether natural or created by humans, sooner or later undergoes alterations as a consequence of the interaction between their structure and environment. Leather can be considered as the first biomaterial ever produced. It was one of the major materials in the past, and its use continues into the present. Its wide usability is the reason why leather artefacts are frequently found during archaeological excavations. The varied properties of leather that provide such a wide use are due to the intrinsic chemical, morphological and physical properties of collagen, hence to the species from which it originates, and to the method with which hide was processed. The vital characteristics of collagen as the main structural protein of most hard and soft tissues in animals and the human body, including thermal stability, mechanical strength and the ability to engage in specific interactions with other biomolecules, lies on its hierarchical structure based on the collagen fibril as an elementary building block [1]. A collagen fibril is a bundle of collagen molecules of approximately 10 to 300 nm in diameter and several micrometres in length. A collagen molecule is composed of three left-handed helical chains, which assemble to form a triple-helix structure [2,3,4,5]. This triple helix, coiled structure is stereo-dynamically favourable to allow strands to be interwoven together and assemble by way of cross-linking in a complex, in a hierarchical manner that ultimately leads to the macroscopic fibres that form extensive and robust networks, providing the dermis with strength, firmness and elasticity. A collagen fibre essentially comprises bundles of smaller fibrils and has a characteristic coiled structure [6]. Through tanning (namely collagen binding with tanning agents), mechanical strength and thermal stability of skins and hides are enhanced allowing their conversion into a durable and manageable material called leather which does not rot in moist condition and withstand wear and tear. Such properties have allowed leather to survive even under extreme condition such as arid waterless areas, waterlogged areas, permafrost, peat bogs etc.

Leather making is a very long process and consists of many different chemical and mechanical process steps [7]. Tanning is the most important step of the whole leather making process. It is commonly performed either by vegetable or mineral tanning. Vegetable tannage with organic materials obtained from different parts of plants including woods, barks, fruits, fruit pods, seeds and leaves left to macerate in water was mostly used up to nineteenth century. Current commercial tanning agents, include mostly mineral (mainly Cr(III) salts) and organic (aldehydes) reagents, while vegetable tannins (polyphenolic tannins) are used for luxury leather [8]. Each of these tannages have different chemistries, vegetable tanning being the least well understood [9]. The most recent tanning theory proposed by Covington [10] is based on the link-lock concept that requires an initial reaction to link the collagen into the surrounding matrix of water and a second reaction component to lock the linked structure together, creating a supramacromolecular structure around the triple helices. According to Covington, only the primary link reaction occurs between plant polyphenols and collagen through multiple hydrogen bonding and some covalent bonding via quinoid species, at the collagen lysine amino group. However, a further stabilisation of the collagen matrix is provided by the substitution of some of the supramolecular water (i.e. dehydration) and interaction of tannins with some of the remaining water.

Although vegetable-tanned leather is a highly durable material, its integrity is influenced by external factors which may alter its physical, chemical and mechanical properties. Yet, physical and chemical properties, but also the aesthetic appeal of leather, gradually deteriorate as a result of interaction with its environment until the loss of the function of the object or even until its total loss. In the case of archaeological leather recoverd from underground, the kinetics of deterioration may also be influenced by the sudden change of the environmental conditions through exposure to the atmosphere. It is known that during burial, most objects will experience a period of rapid and extensive degradation initially followed by a longer period in which the object nearly reaches equilibrium with its environment, then deterioration gradually slows down until the moment of excavation, when exposure to a new environment accelerates the process again [11]. The archaeological leather damage condition should therefore be evaluated with utmost urgency to define the optimal conservation conditions and prioritize possible conservation interventions. However, the few studies on archaeological leather reported so far are dealing with the origin of neolithic leather by microstructural, chemical and isotopic evidence [12], identification of archaeological leather by mass spectrometry (ZooMS) [13], evaluation of archaeological leather through amino acid composition and thermal analysis [14], red stain degradation [15] or linseed oil emulsion used in dressing archaeological leather [16]. On the other hand, some of us have used multi-technical approaches to characterize historical leathers which may be composed from a heterogeneous mixture of tanned collagen (chemically modified collagen showing a leather-like hytdrothermal behaviour), un-tanned collagen (chemically unmodified collagen showing a parchment-like behaviour), gelatinised and amorphous collagen, and deterioration compounds [17,18,19,20].

To facilitate the application of a multi-analytical approach to the study of leather remains which spent prolonged periods of time within the earth, burial tests were performed on newly produced leather from both bovine and ovine hides tanned with bydrolisable and condensed tannins. A combination of techniques such as Fourier transform infrared spectroscopy in attenuated total reflection mode (FTIR-ATR), thermal microscopy (MHT method), differential scanning calorimetry (DSC), thermogravimetry (TG/DTG) and scanning electron microscopy (SEM) was employed to provide the best possible insight into the deterioration pattern of burried leather with special focus on collagen–tannin matrix de-tanning and collagen gelatinisation. This approach targets different structural levels of collagen, from molecules to fibrils and fibres, while bringing the advantage of combining surface and bulk analyses.

Materials and methods

Materials

Four newly manufactured vegetable tanned leathers purchased from the Gara TZL PLUS s.r.o., Otrokovice, Czech Republic were used to perform the burial tests. The possible influence of the animal species (bovine and sheep) and age (calf and cattle) as well as of the tannin type (hydrolysable and condensed) was considered. In Table 1, the symbols of the investigated leather samples are explained: the first letter refers to animal species (C for calf, Ca for cattle and S for sheep), the second one to the tannin type (C for condensed tannin and H for hydrolysable tannin) while the figure represents the burial time (0 for not-buried samples, 1, 2 or 4 for samples buried for 1-year, 2-year and 4-year periods).

Table 1 List of the buried leather samples, with their symbols, animal origin and burial time
Full size table

Leather samples were buried to 50 cm depth, at 454 m altitude, in the western part of Czech Republic, a region characterised by the mild central-European climate [21]. The average annual temperature is (6.6–8.0) °C and the average rainfall per year is (550–650) mm [22]. The soil has a sandy loam structure and neutral pH around 6.5. Sandy loam is a well-drained soil mostly composed of sand (silicon dioxide and calcium carbonate) and clay (hydrous aluminium phyllosilicate minerals). Leather is best preserved in very wet, very dry, frozen or low-oxygen burial environments which protect it against biological activity. On the other hand, leather is poorly preserved on sites where there are seasonal freeze-thaw cycles, the soil is well-drained and oxygenated. The pH of the burial environment will also influence the preservation of leather, a material produced with a pH of about 4.5 to 5.5 to ensure the fats and tannins remain bound in the leather structure. Hence, the soil and climate conditions of our experiments should rapidly degrade the buried samples. The samples were dug up and analysed after 1- and 2-year burial periods. Only the cattle sample was kept buried for a 4-year period. Before performing the analytical measurements, all leather samples were soft brushed and rinsed with jets of deionised water to remove the ingrained soil remains, and dried at room temperature (21 ± 1 °C, 45 ± 5% RH) under light pressure to avoid wrinkling.

Methods

Fourier transform infrared spectroscopy in attenuated total reflection mode (FTIR-ATR)

FTIR-ATR measurements were carried out using an ALPHA FTIR (Bruker Optics, Germany) equipped with a Platinum ATR module. The penetration depth, depending on the wavelength, the refractive indices of ATR crystal typically amount to a few microns (ca. 0.5–3 µm). Spectra were recorded by co-adding of 64 scans in the range from 4000 to 400 cm−1 with a spectral resolution of 4 cm−1. Six diferent replicates were measured on both sides of each sample and averaged before data preprocessing. Opus 7.0 software (Bruker Optics, Germany) was used for the acquisition and processing of the spectra, while their graphic presentation was done with Origin 7.5 software.

Differential scanning calorimetry (DSC)

DSC measurements were performed with a DSC 204 F1 Phoenix instrument (Netzsch, Germany) in nitrogen flow (20 mL/min), in the temperature range (25–280) °C at a scanning rate of 10 K/min, in open aluminium crucibles as previously described [23]. Three measurements were performed using fresh subsamples of about 5 mg for each leather sample.

Thermogravimetry/differential thermogravimetry (TG/DTG)

TG/DTG curves were obtained using a STA 449 C Jupiter instrument (Netzsch, Germany). Leather samples of about 5 mg were heated in open aluminium pans in the temperature range (25–500) °C in nitrogen flow (100 mL/min), at a scanning rate of 10 K/min. The rate v310 (d%Δm/dt) of the first process of thermo-oxidation was calculated at 310 °C from the first derivative of TG curve (DTG curve) [23].

Micro Hot Table (MHT)

MHT measurements were carried out using a homemade MHT equipment consisting of a micro-heating plate (Caloris Group, Romania) controlled by a temperature processor and a stereomicroscope (Leica S4E) assisted by a homemade software F.L.T.K. 1.1.X. for data collection. Samples composed of a few fibres from each side of a leather sample were wetted with demineralised water and kept for 10 min on a microscope slide with concavity to facilitate fibres’ separation. Well separated fibres were then covered with a slide, placed on the heating table and heated at 2 C/min. Their shrinkage activity was observed at 40× magnification. At least two measurements were made for each sample.

Scanning electron microscopy (SEM)

Examination of the leather surface morphology before and after the soil burial tests was performed using a Tescan MIRA3 (Brno, CZ) scanning electron microscope. Samples mounted on carbon stubs and covered by a 20 nm AuPd (80/20) layer were observed at different locations at increasing magnification (from 500 to 50,000×). Only the grain surface of leather samples was AuPd covered. SEM observation was performed at 15 keV accelerating voltage and 15 mm working distance.

pH measurement

Surface pH measurements were carried out according to the ISO 10390 standard [24] using a surface electrode WTW SenTix Sur and a pHmeter Mettler Toledo SevenCompact S220. Each measurement spot (1 × 1 cm2) was damped with a droplet of water using a pipette. After 3 min extraction, the electrode was placed on the spot surface and the pH of the water film was measured. The pH value was obtained as the average between 3 measurements.

Results and discussion

Effects of leather burial on collagen shrinkage activity by MHT method

It was shown that the hydrothermal stability of new, undamaged leather depends on the tannin type, animal species and processing method, and influences leather resistance against deterioration [24,25,26,27]. However, both the effect of animal species and tannin type levels out as the damage progresses and hydrothermal stability becomes increasingly influenced by the mechanism of deterioration. The MHT method was purposely set up for quick and simple evaluation of the hydrothermal stability of historical leather and parchment using very tinny sample. It combines thermal treatment and stereomicroscopy [28] allowing conservators to visualise how collagen fibres contract on heating using an inexpensive equipment, easy to use and providing apparently easy to interpret results [29]. The shrinkage (contraction) activity of collagen fibres is described by a sequence of temperature intervals: no activity–A1–B1–ΔC–B2–A2–complete shrinkage. Interval A is characterized by shrinkage activity in individual fibers, one fibre at a time and with pause in between the individual movements. When shrinkage activity in one fibre is immediately followed by shrinkage activity in another fibre without pause, interval B is reached. Interval ΔC, known as the main shrinkage interval, is defined by the simultaneously and continuously shrinking of at least two fibres. Shrinkage temperature Ts refers to the starting temperature of the main shrinkage interval, while Tfirst and Tlast are defined as the temperatures at which the first and last shrinkage activity of an individual fiber is observed. The total length of the shrinkage process is expressed as ΔT = (Tlast − Tfirst).

In the last two decades, shrinkage temperature has been widely used as a metric for damage in old leather and parchment [23, 30,31,32,33] as well as to study the effects of various ageing or conservation treatments on leather and parchment [34,35,36,37,38]. Some of us have reported how leather hydrothermal stability, expressed as Tf and Ts, and structural cohesivity and heterogeneity, expressed as ΔC and ΔT, respectively, correlates with natural or artificially induced ageing patterns [18, 25, 33].

For the investigated vegetable tanned leathers, the variation of shrinkage parameters with burial times is reported in Fig. 1 where the cumulated (A1 + B1) and (A2 + B2) intervals are illustrated. The most important changes we observed were the following:

  1. i.

    Ts increased for all leather samples, regardless of the type of tannin (hydrolysable or condensed), animal species (calf or sheep) or age (calf and cattle) and duration of treatment, except for the CaC4 sample which no longer shows contraction activity in the main shrinkage interval ΔC.

  2. ii.

    Tf decreased for all samples, except for SC2. A dramatic decrease occurred for the CH leather samples tanned with hydrolysable tannin.

  3. iii.

    The shrinkage interval ΔC generally increased, except for the CaC4 sample.

  4. iv.

    The total shrinkage interval ΔT generally increased as a result of increasing either (A1 + B1) or (A2 + B2) or both intervals.

  5. v.

    The shrinkage interval ΔC disappears for CaC4.

Fig. 1
figure 1

Stacked column charts displaying the shrinking intervals for collagen fibres for the investigated leathers before and after the soil burial tests. The main shrinkage temperatures (Tf, Ts and Tl) and intervals (ΔC and ΔT) are indicated

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The most important change refers to an increase in the degree of leather structural inhomogeneity. The increase in structural inhomogeneity was correlated to the key-steps of leather deterioration: thermal destabilization of chemically modified collagen (collagen–tannin matrix), de-tanning (breakdown of the interactions between collagen and tannin), thermal destabilization of chemically unmodified collagen (de-tanned collagen) and gelatinization [18]. Thus, the length of (A1 + B1) interval was related with formation of pre-gelatinized collagen and gelatin, while de-tanning was associated to the length of (A2 + B2) interval [18]. For example, in the case of CH1 and CH2 leather samples, Tf sharply decreases below 30 °C causing the (A1 + B1) interval lengthening. This could be explained by thermal destabilisation and gelatinisation of collagen microfibres [18, 25, 39] and attributed to the neutral soil pH and humidity which favour de-tanning process. It is known that vegetable tannin fixation on collagen is at minimum at pH 5 and decreases with pH increasing. Once the bonds between collagen and tannin are broken, collagen hydrolyses is promoted by soil moisture, while tannins and their possible degradation compounds are washed out by rainwater. On the other hand, de-tanning is in contrast the Ts increase measured for CH samples, from 62 °C to around 70 °C. In general, thermal stabilisation is related to a stronger attraction between the collagen fibres that makes the fibrous network more cohesive (indicated by a shorter ΔC interval) and less susceptible to gelatinisation.

In the case of CC1 and CC2 leather samples, the increase of structural inhomogeneity is equally due to the increase in the number of collagen fibres showing discrete shrinkage of individual fibres in both (A1 + B1) and (A2 + B2) intervals. Tf values higher than 60 °C preclude the existence of gelatin and pregelatinized collagen fractions. On the other hand, the increase of (A2 + B2) interval might be attributed to collagen de-tanning and formation of multiple collagen–tannin fractions with distinct tanning degree and distinct thermal stability.

CaC1 and CaC2 leather samples behaves similarly to CC1 and CC2, respectively. SC1 leather sample behaves similarly to CC1, too, while the SC2’s behaviour is clearly different in that the (A2 + B2) interval fully disappears. A more rapid thermal destabilisation of the collagen–tannin matrix within sheep leather and a greater propensity to de-tanning during artificial ageing compared to calf leather has already been observed by some of us [25, 39].

Interestingly, for all leather samples, the more coherent collagen fibres (namely those showing shrinkage in the main shrinkage interval ΔC) shown a slight increase of their thermal stability (indicated by the Ts increase), while the length of ΔC interval increased. This behaviour is in contrast to what Larsen et al. [29] and Carsote and Badea [18] observed for most artificially aged and historical leathers, namely a parallel decrease of Ts value and ΔC length as deterioration increases. On the other hand, a parallel increase of Ts and ΔC interval was recently reported for vegetable tanned leather exposed to low-dose gamma irradiation and explained by the formation of molecular cross-links that draws the collagen molecules and fibrils closer together, thus increasing their structural order and thermal stability [40]. In the case of buried leathers, we could infer a mechanical strengthening of collagen fibres through the cementation effect of soil minerals, especially silica, instead of a chemical strengthening (cross-linking), with similar effects on leather shrinkage temperature Ts and ΔC interval. Consequently, the extension of (A1 + B1) and (A2 + B2) intervals should also be partially ascribed to the slowdown of the contraction movements caused by “hardening and welding” of collagen fibres and silica minerals.

Finally, the ΔC disappearance, as for CaC4 sample, has been related to a major damage, i.e., the conversion of collagen ordered structures into coiled structures [38, 39].

Effects of leather burial on collagen thermal stability by DSC

The DSC curves for the new leather samples measured in open crucibles and gas flow (Fig. 2) displayed a broad endotherm ranging from room temperature to about 120 °C, associated with loss of moisture, followed by two small endotherms in the temperature intervals (135–150) °C and (220–240) °C, respectively (Table 2). These two small endotherms are in good agreement with the literature data [41,42,43] and our previous results [44, 45]. Budrugeac et al. [17] assigned these two endotherms to thermal denaturation of the crystalline collagen embedded in the amorphous matrix. Considering the very different thermal stability of the two collagen populations, we attribute the two endotherms to two collagen populations with distinct crystallinity and hydration degree according to the two-phase model of collagen in leather represented by a crystalline phase embedded into an amorphous matrix [43].

Fig. 2
figure 2

DSC curves obtained in open crucibles and nitrogen flow for leather samples before and after the 1-year soil burial: a CH; b CC; c SC and d CaC. The dehydration peak, denaturation peak of the collagen–tannin matrix (showing a maximum at Td1), and denaturation peak of the crystalline fraction (showing a maximum at Td2) are indicated

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Table 2 DSC and TG/DTG parameters of leather samples before and after the soil burial tests: denaturation temperature of the collagen matrix (Td1), denaturation temperature of the crystalline fraction (Td2) and the rate (V310/%min−1) of the first process of thermal oxidation
Full size table

For all buried samples, these small endotherms disappeared after 1-year underground. A similar behaviour, previously observed for parchments exposed to the combined action of polluting gases (50 ppm NOx + SO2) and dry heat (100 °C), was attributed to the full denaturation of both the amorphous and crystalline collagen fractions [45]. This indicates that he burial tests we performed had already a strong denaturing effect on collagen after the first year regardless of the type of tannin or animal species. The DSC results thus confirm that the increased thermal stability determined by MHT method is not real and can only be explained by the mechanical effect of fiber aggregation through soil minerals.

Effects of leather burial on collagen susceptibility to thermo-oxidative deterioration by TG/DTG

It was reported that the non-isothermal deterioration of leather occurs through three processes accompanied by mass loss: (i) water loss at T < 150 °C, (ii) pyrolytic thermo-oxidation and (iii) thermal decomposition of the material [17, 23]. These processes are evidenced in the DTG curves by the peaks I, II and III (Fig. 3). The rate of the thermo-oxidation process corresponds to the maximum of the thermo-oxidation peak, namely the peak II (Fig. 3). For various collagen-based materials it was shown that the rate d%Δm/dt of the pyrolytic thermo-oxidation process calculated at 310 °C well correlates with the collagen matrix cross-linking degree. Accordingly, Budrugeac et al. [17] used this parameter as an indicator of the damage level in historical collagen-based materials relating the high rates of the thermo-oxidation process to not-damaged/well conserved leather, and the lower rates to various damage levels. In fact, the lower the rate the higher the deterioration degree. The rates d%Δm/dt of the pyrolytic thermo-oxidation process for the investigated leather samples reported in Table 2 show a progressive decrease of the oxidation rate as burial time increases. This trend of the thermo-oxidation susceptibility clearly indicates an increase of the damage level with burial time.

Fig. 3
figure 3

TG and DTG curves showing the typical decomposition pattern for a newly manufactured vegetable-tanned leather characterised by three successive exothermic processes: water loss (peak I), pyrolytic thermal oxidation (peak II) and thermal decomposition (peak III)

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Effects of leather burial on molecular alteration of collagen–tannin matrix by FTIR-ATR

FTIR spectroscopic analysis is widely used to characterise the molecular changes in the collagen secondary structure [46,47,48,49,50,51]. Due to its non-invasivity and non-destructivity, FTIR-ATR technique has proved suitable for studying the alterations in the secondary structure of collagen within historical leathers [20, 52] as well as for characterizing their vegetable tannin composition [53, 54]. The following aspects were considered when studying the FTIR-ATR spectra of the investigated leather samples: (i) the effects of burial tests on the complex spectral bands of leather characterised by the overlapping of collagen (amide I and amide II) and vegetable tannin bands; (ii) the identification of other compounds on the leather surface, due to either the fabrication process or burial test.

In Fig. 4, the FTIR-ATR spectra of the buried leather samples, CaC1, CaC2 and CaC4, are illustrated and compared with that of the new leather CaC0. For all leather samples, the main infrared absorption bands of collagen were cleary detected and attributed: (i) the amide A (AA) at ~ 3300 cm−1 related to stretching vibrations of the amide N–H bonds; (ii) the amide B (AB) at ~ 3080 cm−1 attributed to the Fermi resonance overtone of the amide II vibration; (iii) the amide I (AI) at ~ 1633 cm−1 arising mainly from the C=O stretching vibration; (iv) the amide II (AII) at ~ 1538 cm−1 attributed to NH in plane bend and CN stretching vibration and (v) amide III (AIII) at ~ 1235 cm−1 corresponding to the NH bending, CN stretching vibration and small contributions from both CO in plane bending and CC stretching vibration. The presence of lipids typical bands at ~ 2920 and 2850 cm−1 (νasCH2 and νsCH2) and at ~ 1735 (νasC=O) could be ascribed to the fatliquoring products used in the leather finishing process to impart softness and flexibility to leather. Worthy of note, these bands show a significant intensity reduction in the CaC4 spectrum indicating the loss of fatliquoring oils which are water-soluble and susceptible to oxidation. Oils leaching makes leather more vulnerable to hydrolysis and to losing its collagenous ordered structure. This finding well correlates with the lack of the main shrinking interval in case of CaC4 sample. Soiling effects are indicated by the aluminosilicates specific bands at ~ 1030 cm−1 (νSi–O), 525 (δAl–O–Si) and 465 cm−1 (δSi–O–Si), as well as by the calcium carbonate main absorption bands at 1405 cm−1 (νasCO3) and 874 cm−1 (δCO3).

Fig. 4
figure 4

FTIR-ATR spectra for CaC, before and after the soil burial tests. The main absorption bands of collagen (AA, AB, AI, AII and AIII), as well as those corresponding to calcium carbonate (1405 and 874 cm−1), fatliquoring oils/fats (2920, 2850 and 1735 cm−1) and aluminosilicates (1030, 525 and 465 cm−1) are highlighted

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To discern the peak positions in the overlapping IR bands of collagen and tannins, the second derivatives of FTIR-ATR spectra were calculated in the spectral range of amide I and amide II (1700–1450 cm−1) for all the investigated samples (Fig. 5a–d). The overlapping spectral bands were assigned to the following components: amide I components centered near 1660 cm−1 and 1622–1628 cm−1, respectively; amide II band observed near 1550 cm−1; vegetable tannins bands near 1602–1605 cm−1 (visible as a shoulder of the second component of amide I) and 1502–1515 cm−1 (this band completely overlap the second component of amide II). Previously, amide I band at about 1658 cm−1 has been reported for native collagen, while the bands at 1651 cm−1 and 1633 cm−1 were correlated with denatured collagen and gelatin, respectively [55]. Similarly, the amide II components at lower wavelengths have been associated to collagen disordered structures (i.e., the band near 1530 cm−1) [48, 52] and gelatin (i.e., the band near 1518 cm−1) [38, 48].

Fig. 5
figure 5

The second derivative FTIR-ATR spectra in the (1700–1450) cm−1 region for a CH; b CC; c SC and d CaC, before and after soil burial tests. Figures in black reffer to collagen band's wavelengths, while figures in red reffer to tannin‘s bands wavelengths

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As a result of the burial tests, the intensity of both amide I components decreased, suggesting a loosening of the collagen–tannin interactions (Figs. 5a–d). This destabilization process led to the dissappearance of the shoulder at 1602–1605 cm−1 after the second year, most likely due to a de-tanning process. The loosening and then breaking of collagen–tannin interactions during burial is also suported by the changes in the amide II band, namely the shift of the amide II component at 1550 cm−1 (α-helix) towards lower wavenumbers (1546–1538 cm−1) accompanied by a progressive depletion of the complex band at 1502–1515 cm−1 [38, 52, 55]. In case of CH leather samples, the intensity of the 1660 cm−1 component (α-helix) decreased while the intensity of the 1628 cm−1 component (gelatine) increased [37, 38, 40, 55]. This behaviour, explained by the unfolding of the native triple helix structure and conversion to gelatin, confirms the gelatinisation of CH leather revealed by the dramatic decrease of Tf evidenced through the MHT method. A similar spectral behaviour was previosly explained by Stani et al. [51] through the formation of a larger number of water-mediated hydrogen bonds in denatured collagen. They assigned the component near 1630 cm−1 mainly to the carbonyl stretching of hydroxyproline and of the others amino acids involved in water mediated hydrogen bonds in the native state. Recently, some of us related the concomitant increase in intensity of amide I component at 1632 cm−1 and depletion of amide II component near 1549 cm−1 to collagen gelatinisation as a result of parchment dry heating [38].

Effects of burial tests on leather surface morphology by SEM

Scanning electron microscopy (SEM) is an invasive but non-destructive high-resolution technique used for imaging a wide range of materials including the collagenous materials. Depending on magnification, changes in the surface microstructure of leather, i.e. the morphology of fibre network, fibres and microfibrils, can be observed as a result of either natural or induced deterioration [56,57,58].

The collagen structural damage documented by SEM imaging of both new and buried samples are illustrated in Figs. 6, 7 and 8. The newly manufactured leather showed ordered fibrillar networking with the fibrils grouped in bundles (Fig. 6a) and their typical periodic structure cleary visible at higher magnification (Fig. 7a). The buried samples displayed discontinuities and loosening of the fibre matrix cohesion, progresive shrinkage and extended melted/gelatinised areas (Fig. 6b, c). The distorted fibrilar structure and massive presence of amourphous and gelatinised structures are already evident at higher magnification after 1-year burial period (Fig. 7b). Bozec and Odlyha [59] related the loss of the repeted periodicity of collagen fibril ultrastructure to collagen entirely structurally denaturated. This is in accordance with the DSC results presented above.

Fig. 6
figure 6

SEM images showing a the ordered fibrillar networking with the fibriles grouped in bundles for CaC0; b, c loss of the fibre matrix cohesion, progresive shrinkage and melted/gelatinised areas for CaC1 and CaC2. Magnification 5000×

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Fig. 7
figure 7

SEM images showing a the typical periodic structure of the collagen fibril for CaC0 and b complete loss of the surface fibrillar structure for CaC1. Magnification 50,000×

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Fig. 8
figure 8

SEM images of CC1 a at 5000× magnification and b at 15,000× magnification showing the presence of CaCO3 crystals (1), bacterial spores (2) and filaments (3) resembling the typical structure of Actinomycetes

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In addition, a microbial attack with the leaching of mineral component on the leather surface (most problably calcium carbonate, as indicated by FTIR-ATR results) was observed (Fig. 8). The chains of bacterial spores and filaments resemble the typical structure of some Actinomycetes [58, 60], aerobic spore forming gram-positive bacteria, which are the most abundent organism that form thread-like filaments in the soil and play a major rôle in the cycling of organic matter [61].

pH variation as a result of the burial tests

The pH value of the new vegetable tanned leathers was in the range 3.5–4.6, depending on the tannin type and manufacturing process. The pH values of the buried leather increased to 6.5–7.3, which is actually the pH value of the soil. In fact, the soil acts as a buffer, so the pH value of the archaeological leather does not depend on the type of damage, but on the type of soil in which it was buried. In the burial experiment we performed, the pH increase facilitated de-tanning of collagen–tannin matrix, thus promoting the subsequent deterioration of collagen until complete denaturation and gelatinization [18]. In addition, at pH > 6, the leaching out of vegetable tannins is favoured. They are easily washed out by rainwater thus speeding up de-tanning process.

Conclusions

This work aimed to study the deterioration induced in vegetable leather by ground burial for 1 to 4 years. For the first time, burial tests were applied on new leather obtained from both bovine and ovine hides using both hydrolysable and condensed tannins. The analytical approach based on FTIR-ATR, DSC, TG/DTG, MHT and SEM provided clear evidences that could help understanding deterioration of archaeological leather.

The MHT method shown a general trend of increasing the structural inhomogeneity degree of collagen within the microfibres. The lengthening of (A1 + B1) interval was correlated with collagen pre-gelatinization and gelatin formation while de-tanning was the main cause of the (A2 + B2) interval lengthening. However, it must be taken into account that the length of these intervals is partly due to fibre hardening/cementing through soil mineral penetration into leather porous structure. This also causes an artificial increase of Ts. Hence, the use of shrinkage temperature as a metric of deterioration might be highly misleading. Noteworthy, the MHT method is still suitable for detecting pregelatinized/gelatinized collagen microfibres being thus essential for deciding the conservation interventions immediately after leather excavation. We also found that leather samples tanned with hydrolysable tannin suffered a much faster de-tanning process at the neutral pH of the soil, while sheep leather shown a sudden thermal destabilisation after the second year underground.

DSC analysis revealed a strong denaturing effect on collagen by the disappearance of both the denaturation peaks corresponding to the two collagen populations with distinct thermal stability, crystallinity and hydration degree usually detected in the temperature interval (135–240) °C. TG/DTG results indicated the progressive reduction of cross-linking degree in the collagen–tannin matrix for all leather samples.

The second derivative of the FTIR-ATR leather spectra allowed us to differentiate between collagen and tannin spectral components and detect their individual shifts and relative intensity variation induced by the burial test. Thus, the de-tanning process (namely, loosening and breakdown of the collagen–tannin interactions) is indicated by the depletion of the tannin bands at 1602–1605 cm−1 and 1502–1515 cm−1, accompanid by the shift of the amide II component at 1550 cm−1 towards lower wavenumbers (1546–1538 cm−1). The conversion of collagen into gelatin is suggested by the decrease of 1660 cm−1 band (assigned to α- helical structures) and increase of the 1628 cm−1 band (assigned to gelatine).

SEM images provided useful information concerning the loosening of the fibre matrix cohesion, progresive shrinkage and formation of melted/gelatinised areas. The distorted collagen fibril ultrastructure well correlated with collagen denaturation and gelatinisation. In addition, the detection of bacterial spores and filaments revealed a microbial atack with the leaching of calcium carbonate crystals (also detected by FTIR-ATR) on leather surface.

Overall, our results confirm that damage in buried leathers follows the same main steps identified in historical leather long-term deterioration, namely leather de-tanning, collagen denaturation and gelatinisation [18]. These findings improved our understanding of the burial-induced deterioration in vegetable tanned leather and could be of great assistence in assessing archaeological leather. The analytical protocol based on MHT, DSC, FTIR-ATR and SEM demonstrated a good potential as a routine approach for a comprehensive characterization of collagen matrix structure and stability from molecular to macroscopic level. Moreover, a correct interpretation of the shrinkage activity of archaelogical leathers could offer enough information for a quick and handy characterization of leather structural stability.

Availability of data and materials

The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.

Abbreviations

T:

Temperature

TG:

Thermal gravimetry

DTG:

Differential thermal gravimetry

DSC:

Differential scanning calorimetry

MHT:

Micro Hot Table

FTIR:

Fourier transform infrared spectroscopy

ATR:

Attenuated total reflection

SEM:

Scanning electron microscopy

References

  1. Fratzl P. Collagen: structure and mechanics, an introduction. In: Fratzl P, editor. Collagen. Berlin: Springer; 2008. p. 1–13. https://doi.org/10.1007/978-0-387-73906-9_1.

    Chapter  Google Scholar 

  2. Rich A, Crick FHC. The molecular structure of collagen. J Mol Biol. 1961;3:483–506.

    Article  CAS  Google Scholar 

  3. Shoulders MD, Raines RT. Collagen structure and stability. Annu Rev Biochem. 2009;78:929–58.

    Article  CAS  Google Scholar 

  4. Orgel JPRO, Irving TC, Wess TJ. Microfibrillar structure of type I collagen in situ. Proc Natl Acad Sci USA. 2006;103:9001–5.

    Article  CAS  Google Scholar 

  5. Persikov AV, Ramshaw JA, Brodsky B. Prediction of collagen stability from amino acid sequence. J Biol Chem. 2005;280:19343–9.

    Article  CAS  Google Scholar 

  6. Buehler MJ. Nature designs tough collagen: explaining the nanostructure of collagen fibrils. Proc Natl Acad Sci USA. 2006;103:12285–90.

    Article  CAS  Google Scholar 

  7. Covington AD. Modern tanning chemistry. Chem Soc Rev. 1997;26:111–26.

    Article  CAS  Google Scholar 

  8. Covington AD. Tanning chemistry: the science of leather. In: Wise W, Covington AD, editors. Cambridge: The Royal Society of Chemistry; 2019.

  9. Schröpfer M, Meyer M. Investigations towards the binding mechanisms of vegetable tanning agents to collagen. Res J Phytochem. 2016;10(2):58–66. https://doi.org/10.3923/rjphyto.2016.58.6.

    Article  Google Scholar 

  10. Covington AD, Song L, Suparno O, Koon H, Collins MJ. Link-lock: an explanation of the chemical stabilisation of collagen. J Soc Leather Technol Chem. 2008;92:1–7.

    CAS  Google Scholar 

  11. Newton C, Cook C. Caring for archaeological collections. Preventive conservation guidelines for collections. Government of Canada, Canadian Conservation Institute. https://www.canada.ca/en/conservation-institute/services/preventive-conservation/guidelines-collections.html.

  12. Spangenberg JE, Ferrer M, Tschudin P, Volken M, Hafner A. Microstructural, chemical and isotopic evidence for the origin of late neolithic leather recovered from an ice field in the Swiss Alps. J Archaeol Sci. 2010;37(8):1851–65. https://doi.org/10.1016/j.jas.2010.02.003.

    Article  Google Scholar 

  13. Ebsen JA, Haase K, Larsen R, Poulsen Sommer DV, Ørsted BL. Identifying archaeological leather—discussing the potential of grain pattern analysis and zooarchaeology by mass spectrometry (ZooMS) through a case study involving medieval shoe parts from Denmark. J Cult Herit. 2019;39:21–31. https://doi.org/10.1016/j.culher.2019.04.008.

    Article  Google Scholar 

  14. Plavan V, Miu L, Gavrilyuk N. Evaluation of the amino acid composition, structure and properties of archaeological leather. Procedia Chem. 2013;8:279–83. https://doi.org/10.1016/j.proche.2013.03.034.

    Article  CAS  Google Scholar 

  15. Koochakzaei A, Achachluei MM. Red stains on archaeological leather: degradation characteristics of a shoe from the 11th–13th centuries (Seljuk period, Iran). J Am Inst Conserv. 2015;54(1):45–56. https://doi.org/10.1179/1945233014Y.0000000033.

    Article  Google Scholar 

  16. Hassan RRA. A preliminary study on using linseed oil emulsion in dressing archaeological leather. J Cult Herit. 2016;21:786–95.

    Article  Google Scholar 

  17. Budrugeac P, Carsote C, Miu L. Application of thermal analysis methods for damage assessment of leather in an old military coat belonging to the History Museum of Braşov-Romania. J Therm Anal Calorim. 2017;127(1):765–72. https://doi.org/10.1007/s10973-016-5343-8.

    Article  CAS  Google Scholar 

  18. Carsote C, Badea E. Micro differential scanning calorimetry and Micro Hot Table method for quantifying deterioration of historical leather. Herit Sci. 2019;7(48):1–13. https://doi.org/10.1186/s40494-019-0292-8.

    Article  Google Scholar 

  19. Carsote C, Badea E, Caniola I, Paunescu S, Lupas MC, Sendrea C, Miu L. The Homiliary of Varlaam: scientific investigation of the leather bookbinding. Rev Chim. 2020;71(3):51–8. https://doi.org/10.37358/RC.20.3.7973.

    Article  CAS  Google Scholar 

  20. Proietti N, Di Tullio V, Carsote C, Badea E. 13C solid-state NMR complemented by ATR-FTIR and micro-DSC to study modern collagen-based material and historical leather. Magn Reson Chem. 2020;58:840–59. https://doi.org/10.1002/mrc.5024.

    Article  CAS  Google Scholar 

  21. Geoportal. The Czech National Geoportal. 2019. https://geoportal.gov.cz/web/guest/map?permalink=3ce4d8ab3c6bc1ee95a090c4f411668f.

  22. CHMI. Czech Hydrometeorological Institute. 2020. http://portal.chmi.cz/historicka-data/pocasi/uzemni-srazky?l=en.

  23. Budrugeac P, Cucos A, Miu L. The use of thermal analysis methods for authentication and conservation state determination of historical and/or cultural objects manufactured from leather. J Therm Anal Calorim. 2011;104:439–50.

    Article  CAS  Google Scholar 

  24. ISO, Č., ČSN ISO 10390: soil quality—determination of pH. Český normalizační institut. 2011. p. 12.

  25. Badea E, Şendrea C, Carsote C, Adams A, Blümich B, Iovu H. Unilateral NMR and thermal microscopy studies of vegetable tanned leather exposed to dehydrothermal treatment and light irradiation. Microchem J. 2016;129:158–65. https://doi.org/10.1016/j.microc.2016.06.013.

    Article  CAS  Google Scholar 

  26. Sebestyén Z, Jakab E, Badea E, Barta-Rajnai E, Şendrea C, Czégény Z. Thermal degradation study of vegetable tannins and vegetable tanned leathers. J Anal Appl Pyrol. 2019;138:178–87. https://doi.org/10.1016/j.jaap.2018.12.022.

    Article  CAS  Google Scholar 

  27. Chahine C. Changes in hydrothermal stability of leather and parchment with deterioration: a DSC study. Thermochim Acta. 2000;365:101–10.

    Article  CAS  Google Scholar 

  28. Larsen R, Vest M, Nielsen K. Determination of hydrothermal stability (shrinkage temperature) of historical leathers by Micro Hot Table technique. J Soc Leather Technol Chem. 1993;77:151–6.

    CAS  Google Scholar 

  29. Larsen R, Vest M, Nielsen K. Determination of hydrothermal stability. In: Larsen R, Vest M, Kejser UB, editors. STEP Leather Project. Evaluation of the correlation between natural and artificial ageing of vegetable tanned leather and determination of parameters for standardization of an artificial ageing method. Copenhagen: Bjarnholt Repro; 1994. p. 151–64.

    Google Scholar 

  30. Larsen R, Poulsen Sommer DV, Mühlen AK. Scientifc approach in conservation and restoration of leather and parchments objects in archives and libraries. In: Engel P, editor. New approaches to book and paper conservation-restoration. Wien: Verlag Berger; 2011. p. 239–58.

    Google Scholar 

  31. Larsen R. Experiments and observations in the study of environmental impact on historical vegetable tanned leathers. Thermochim Acta. 2000;365:85–99.

    Article  CAS  Google Scholar 

  32. Carsote C, Kövari L, Albu C, Hadîmbu E, Badea E, Miu L, Dumitrescu G. Bindings of rare books from the collections of the Romanian Academy Library—a multidisciplinary study. Leather Footwear J. 2018;18(4):307–20.

    Article  CAS  Google Scholar 

  33. Badea E, Poulsen Sommer DV, Muhlen Axelsson K, Larsen R, Kurysheva A, Miu L, Della Gatta G. Damage ranking of historic pachments: from microscopic studies of fibre structure to collagen denaturation assessment by micro DSC. e-Preserv Sci. 2012;9:97–109.

    CAS  Google Scholar 

  34. Vyskočilová G, Ebersbach M, Kopecká R, Prokeš L, Příhoda J. Model study of the leather degradation by oxidation and hydrolysis. Herit Sci. 2019;7:1–13. https://doi.org/10.1186/s40494-019-0269-7.

    Article  Google Scholar 

  35. Badea E, Carsote C, Hadimbu E, Sendrea C, Lupas MC. The effect of halloysite nanotubes dispersions on vegetable-tanned leather thermal stability. Herit Sci. 2019;7:68. https://doi.org/10.1186/s40494-019-0310-x.

    Article  CAS  Google Scholar 

  36. Sendrea C, Carsote C, Radu M, Badea E, Miu L. The effect of gamma irradiation on shrinkage activity of collagen in vegetable tanned leather. Rev Chim. 2017;68:1535–8. https://doi.org/10.37358/rc.17.7.5711.

    Article  CAS  Google Scholar 

  37. Cappa F, Paganoni I, Carsote C, Badea E, Schreiner M. Studies on the effects of mixed light-thermal ageing on parchment by vibrational spectroscopy and micro hot table method. Herit Sci. 2020;8(15):1–12. https://doi.org/10.1186/s40494-020-0353-z.

    Article  Google Scholar 

  38. Cappa F, Paganoni I, Carsote C, Schreiner M, Badea E. Studies on the effect of dry-heat ageing on parchment deterioration by vibrational spectroscopy and micro hot table method. Polym Degrad Stab. 2020;182(109375):1–11. https://doi.org/10.1016/j.polymdegradstab.2020.109375.

    Article  CAS  Google Scholar 

  39. Carsote C, Badea E, Miu L, Della GG. Study of the effect of tannins and animal species on the thermal stability of vegetable leather by differential scanning calorimetry. J Therm Anal Calorim. 2016;124:1255–66. https://doi.org/10.1007/s10973-016-5344-7.

    Article  CAS  Google Scholar 

  40. Carsote C, Şendrea C, Micu MC, Adams A, Badea E. Micro-DSC, FTIR-ATR and NMR MOUSE study of the dose-dependent effects of gamma irradiation on vegetable-tanned leather: the influence of leather thermal stability. Radiat Phys Chem. 2021;189:109712. https://doi.org/10.1016/j.radphyschem.2021.109712.

    Article  CAS  Google Scholar 

  41. Miles CA, Ghelashvili M. Polymer-in-a-Box Mechanism for the thermal stabilization of collagen molecules in fibers. Biophys J. 1999;78(6):3243–52.

    Article  Google Scholar 

  42. Samouillan V, Delaunay F, Dandurand J, Merbahi N, Gardou JP, Yousfi M, Gandaglia A, Spina M, Lacabanne CJ. The use of thermal techniques for the characterization and selection of natural biomaterials. Funct Biomater. 2011;2:230–48. https://doi.org/10.3390/jfb2030230.

    Article  CAS  Google Scholar 

  43. Popescu C, Budrugeac P, Miu L, Wortmann FJ, Demco DE, Baias M. Assessment of collagen-based materials which are supports of cultural and historical objects. Polym Degrad Stabil. 2008;93(5):976–82.

    Article  CAS  Google Scholar 

  44. Budrugeac P, Badea E, Della Gatta G, Miu L, Comanescu A. A DSC study of deterioration caused by environmenthal chemical pollutants to parchment, a collagen-based material. Thermochim Acta. 2010;500:51–62.

    Article  CAS  Google Scholar 

  45. Badea E, Usacheva T, Della GG. The use of differential scanning calorimetry to characterise collagen deterioration in parchment. Russ Chem J. 2015;59(1):28–41.

    Google Scholar 

  46. Doyle BB, Bendit EG, Blout ER. Infrared spectroscopy of collagen and collagen-like polypeptides. Biopolymers. 1975;14:937–57. https://doi.org/10.1002/bip.1975.360140505.

    Article  CAS  Google Scholar 

  47. Payne KJ, Veis A. Fourier transform IR spectroscopy of collagen and gelatin solutions: deconvolution of the amide I band for conformational studies. Biopolymers. 1988;27:1749–60. https://doi.org/10.1002/bip.360271105.

    Article  CAS  Google Scholar 

  48. Barth A. Infrared spectroscopy of proteins. Biochim Biophys Acta Bioenerg. 2007;1767:1073–101. https://doi.org/10.1016/j.bbabio.2007.06.004.

    Article  CAS  Google Scholar 

  49. De Campos VB, Mello MLS. Collagen type I amide I band infrared spectroscopy. Micron. 2011;42:283–9. https://doi.org/10.1016/j.micron.2010.09.010.

    Article  CAS  Google Scholar 

  50. Glassford SE, Byrne B, Kazarian SG. Recent applications of ATR-FTIR spectroscopy and imaging to proteins. Biochim Biophys Acta. 2013;1834:2849–58.

    Article  CAS  Google Scholar 

  51. Stani C, Vaccari L, Mitri E, Birarda G. FTIR investigation of the secondary structure of type I collagen: new insight into the amide III band. Spectrochim Acta A Mol Biomol Spectrosc. 2020;229:118006.

    Article  CAS  Google Scholar 

  52. Vichi A, Eliazyan G, Kazarian SG. Study of the degradation and conservation of historical leather book covers with Macro Attenuated Total Reflection-Fourier Transform Infrared Spectroscopic Imaging. ACS Omega. 2018;3:7150–7.

    Article  CAS  Google Scholar 

  53. Falcão L, Araújo MEM. Tannins characterization in historic leathers by complementary analytical techniques ATR-FTIR, UV-Vis and chemical tests. J Cult Herit. 2013;14:499–508.

    Article  Google Scholar 

  54. Falcão L, Araújo MEM. Vegetable tannins used in the manufacture of historic leathers. Molecules. 2018;23:8–10. https://doi.org/10.3390/molecules23051081.

    Article  CAS  Google Scholar 

  55. Rabotyagova OS, Cebe P, Kaplan DL. Collagen structural hierarchy and susceptibility to degradation by ultraviolet radiation. Mater Sci Eng C. 2008;28:1420–9. https://doi.org/10.1016/j.msec.2008.03.012.

    Article  CAS  Google Scholar 

  56. Bicchieri M, Biocca P, Colaizzi P, Pinzari F. Microscopic observations of paper and parchment: the archaeology of small objects. Herit Sci. 2019;7(47):1–12. https://doi.org/10.1186/s40494-019-0291-9.

    Article  Google Scholar 

  57. Vadrucci M, Cicero C, Parisse P, Casalis L, De Bellis G. Surface evaluation of the effect of X-rays irradiation on parchment artefacts through AFM and SEM. App Surf Sci. 2020;513:145881. https://doi.org/10.1016/j.apsusc.2020.145881.

    Article  CAS  Google Scholar 

  58. Piñar G, Sterflinger K, Pinzari F. Unmasking the measles-like parchment discoloration: molecular and microanalytical approach. Environ Microbiol. 2015;17(2):427–43. https://doi.org/10.1111/1462-2920.12471.

    Article  CAS  Google Scholar 

  59. Bozec L, Odlyha M. Thermal denaturation studies of collagen by microthermal analysis and atomic force microscopy. Biophys J. 2011;101:228–36.

    Article  CAS  Google Scholar 

  60. Pinzari F, Gutarowska B. Extreme colonizers and rapid profiteers: the challenging world of microorganisms that attack paper and parchment. In: Joseph E, editor. Microorganisms in the deterioration and preservation of cultural heritage. Berlin: Springer; 2021. https://doi.org/10.1007/978-3-030-69411-1_4.

    Chapter  Google Scholar 

  61. Bhatti AA, Haq S, Bhat RA. Actinomycetes benefaction role in soil and plant health. Microb Pathog. 2017;111:458–67. https://doi.org/10.1016/j.micpath.2017.09.036.

    Article  CAS  Google Scholar 

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Acknowledgements

Gabriela Vyskočilová is grateful to Dr. Lucretia Miu for hosting her in the ARCH Lab of National Research & Development Institute for Textile and Leather during her Erasmus research stage.

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This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic

    Gabriela Vyskočilová & Richard Ševčík

  2. National Museum of Romanian History, Bucharest, Romania

    Cristina Carşote

  3. Advanced Research for Cultural Heritage Group (ARCH Lab), National Research & Development Institute for Textile and Leather, ICPI Division, Bucharest, Romania

    Elena Badea

  4. Department of Chemistry, Faculty of Sciences, University of Craiova, Craiova, Romania

    Elena Badea

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Contributions

GV: methodology, FTIR-ATR investigation, MHT investigation, SEM investigation, TG/DTG investigation, DSC investigation, validation, writing—original draft. RS: methodology, supervision. CC: data analysis, validation, writing—review and editing. EB: data analysis, validation, supervision, writing—review and editing. All authors read and approved the final manuscript.

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Correspondence to Elena Badea.

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Vyskočilová, G., Carşote, C., Ševčík, R. et al. Burial-induced deterioration in leather: a FTIR-ATR, DSC, TG/DTG, MHT and SEM study. Herit Sci 10, 7 (2022). https://doi.org/10.1186/s40494-021-00638-6

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