Statistics of insect catch within historic properties
© Brimblecombe et al.; licensee Chemistry Central Ltd. 2013
Received: 30 June 2013
Accepted: 29 August 2013
Published: 8 November 2013
Certain species of insect are known to cause damage to historic collections. For more than a decade insects have been identified in traps set out in English Heritage properties, the data from which has been used in this paper. Descriptive statistics have been used to explore the data structure and insect distribution.
About 55% of the traps of the more than 30000 traps examined over that period contained insects that were readily categorised. The rate of catch (insects/trap) was highest in London and the Southeast. Booklice (Liposcelis bostrychophila) and silverfish (Lepisma saccharina) were the most frequent catch. Woolly bear (Anthrenus spp.) larvae and crustacean woodlice (Porcellio spinicornis) were also common. A higher frequency of furniture beetles (Anobium punctatum) is notable in the Southeast and West. Despite this overall pattern, catch varied greatly between individual properties. The general view that insects have increased over time was not universal, although the dominant booklouse showed an increased catch over the last decade. The insects did not appear to be randomly distributed, but clustered onto traps in greater numbers than might be expected from a Poisson distribution, which suggested they occurred as infestations. Some insect species appeared in combination with other species at a higher frequency than expected, but the reasons for these associations were not always obvious. Pheromone traps collected more webbing and case bearing clothes moths (Tineola bisselliella, Tinea pellionella) than traps without attractants, much as expected. There are hints that covered traps may collect fewer insects than simple blunder traps.
No obvious drivers of high insect count were identified. Trends over time were not especially clear. The results provide clues to management of insect pests in historic properties. The presence and trapping of insects at a given property is individual in nature, and so mitigation strategies need to be site-specific. Although an insect might occur in low numbers in some periods, the possibility of infestation remains. Future work will examine the trends in catch more carefully and ascertain the importance of various environmental factors.
Insects have long been a problem because of the damage they can cause. In classical times there was discussion of the damage to clothes by moths, and of potential solutions to this nuisance. Insect pests attack furniture and textiles as well as the structure of wooden buildings, which is of great concern in historic properties. Properties that are no longer occupied offer insects an opportunity to occupy quiet habitats. Especially damaging to historic materials, are the larval stages of carpet beetle (Anthrenus spp.) and clothes moths (Lepidoptera: Tineidae), which feed on textiles, fur and leather, beetles that attack wood and furniture, and silverfish (Lepisma saccharina) and woodlice (Porcellio spp.) that damage books and wallpaper .
A general view has been expressed among some professionals in the heritage conservation field that there may be an increase in insect populations, with a move of more temperature-sensitive species northwards [2, 3]. An example is the barkfly (order Psocoptera, which includes booklice) studied within the National Barkfly Recording Scheme (Britain and Ireland); a species (Atlantopsocus adustus) characteristic of Madeira and the Canary Islands has now been discovered in Cornwall . Awareness of the impact of a warmer climate has grown in recent years, along with concerns about its influence on the appearance of new species in historic collections. Their discovery may also arise from greater movement of objects between collections, which transports insects between properties. There is also the potential impact of changes in food , wider use of natural fabrics, and a more cautious approach to the use of pesticides .
The work presented here uses simple descriptive statistics to explore more than a decade of insect trapping data from properties managed by English Heritage (EH), which was used to get a greater understanding of the distribution and changes in insects populations. Although this paper refers to insects, some of the catch identifies not only insects, so for example woodlice, which are isopods belonging to the crustacea, are frequently logged in large numbers on the traps. The colloquial use of the term bugs (formally Hemiptera) or the term arthropods might better describe the subject of this work, but in the end we have accepted the word “insect” even though it is slightly inaccurate, it seems to capture what most readers will search for in a title.
The research also considered environmental and indoor habitat changes as possible explanations for the insect populations that are observed. However, it takes a “black box” approach, treating the insects caught and the properties evaluated in statistical terms, which limits the interpretation. This should allow strategic analysis, but the results from more focussed research is the subject of subsequent work. Ultimately an improved understanding of these issues should contribute to Integrated Pest Management strategies, although previous assessments of catch have often been limited to the contents of a limited number of traps e.g. . Additionally there is relatively little guidance for the interpretation of insect catches from historic properties e.g. . This study will provide a background against which changes might be measured, especially in studies concerned with the possibility that change is driven by an increasingly warm English climate .
Insects allocated to types and total number of individual species found in traps in order of abundance; where a species is not defined, it is shown in parenthesis
Varied carpet beetle(a)
Webbing clothes moth(a)
Brown house moth
Two spot carpet or fur beetle(l)
Pentarthrum and Euophryum
Case bearing clothes moth(l)
Case bearing clothes moth(a)
White shouldered house moth
Two spot carpet or fur beetle(a)
Webbing clothes moth(l)
Golden spider beetle
White marked spider beetle
Guernsey Carpet beetle
Australian spider beetle
Hide or leather beetle
List of key properties with sufficient sample data
Marble Hill House
Atcham Store 26
Atcham Store 30
Atcham Store 31
Although some 32000 traps were placed, a substantial proportion were lost or damaged. More than 500 traps were listed as missing, damaged or unreadable, and so were removed from the dataset before analysis. This prevented confusion with traps which were examined, but free of any of the listed insects (Table 1). Some 54% of the traps contained insects that were categorised and about 10% of the traps had other observations such as trapped flies, presence of predators, frass etc. Table 1 shows booklice, silverfish and woodlice to be the most abundant. The woolly bear larvae (typically the larva of the varied carpet beetle, Anthrenus verbasci and the Guernsey carpet beetle, Anthrenus sarnicus) and clothes moths were also found frequently.
Among the traps placed out there were more than 550 that contained pheromones to attract either the webbing clothes moth (Tineola bisselliella) or the case bearing clothes moth (Tinea pellionella). There are observations from more than 29600 of the simpler sticky traps, and it is these that form the basis of much of the discussion here. The data is difficult to interpret and gain an overall sense of distribution or change. On the simplest level it exists on almost 1500 individual spreadsheets collected for management purposes over more than ten years. The data contained on the individual spreadsheets were transformed to a single ASCII file for each territory, which could then be read by a wide range of software.
The use of MS Access, Oracle, other SQL databases, or a single spreadsheet to record and store the source data would be efficient approaches, and would eliminate the need to translate the data in this way. Much of the initial processing was done with the simple UNIX script processing language awk, which had the advantage of supporting regular expressions that could interpret property and insect names or alphabetic codes that were entered into fields of the files. The data is unevenly distributed across the territories and through time. It was also important to consider the extent to which insects might have been misidentified or incorrectly entered into columns of the database, although this seems to smaller than other errors, such as miscounting when the catch becomes large. Entry of numbers into incorrect columns can arise at a number of stages from typing the sheets through to the conversion into ASCII code, also complicated by changes to the number and descriptions of the columns and rows used in the sheets over time. The entry of spurious comments which can push numbers into different columns has been a special problem, so considerable effort went into re-inspection of the original sheets to eliminate these incorrect entries. Future trap reporting would benefit from standard methods for recording and storing large amounts of data, consistent terminology, and more stringent controls on the content of cells.
Applying statistical operations to insect catch can be troublesome because just a small integer number is typically recorded per trap. The distribution is highly skewed. Nevertheless we have retained the mean as a measure of central tendency, as the median and mode were often zero for many insects. We do not mark standard deviation in the figures as this can also be misleading for highly skewed distributions. In the cases where we need to express correlation we adopt the Kendall τ rank correlation coefficient along with its two-sided p-value as an estimate of significance using the online Wessa.net web-enabled applications (http://www.wessa.net/). Traditional statistical tests of hypothesis such as the parametric Student’s t-test, were avoided in favour of the non-parametric Mann–Whitney U test in Wessa.net at the section from Statistics Education at Aston University (http://www.wessa.net/aston_university.wasp). The slopes of lines for the time series analysis was taken as a Theil-Sen slope and using the tool provided by Single Case Research (http://www.singlecaseresearch.org/calculators/theil-sen). The Theil-Sen slope is distribution free, and is efficient with non-normal and skewed data. It can be thought of as the median slope. The Chi-squared test was used, but adopted with caution as it was likely to be affected by the large and sometimes uneven sample sizes.
Results and discussion
An initial inspection showed frequent occurrence of numbers such as 20, 30, 100 when the catch became large, possibly due to the difficulty in counting very small insects such as booklice, or when a trap has become saturated with insects. This is likely to overestimate the catch of abundant insects in traps that have become saturated, an error which is most pronounced with booklice. However, these are not the most harmful of insect pests, so it is not likely to lead to an over-reaction in terms of pest management.
Geographical spread of catch
In our study the catch rate is highest in London and the Southeast (Figure 3a), and the percentage of traps that had an identified insect present (% occupancy) follows a similar pattern (Figure 3b), although London dominates with the largest prevalence of occupied traps. London properties have high visitor flow and are open year round, so there is an elevated potential to introduce pests or foodstuff. The properties also exchange and loan collections more frequently than elsewhere. Some of the London houses have experienced problems with damp, and it is always an issue to maintain guttering, chimneys and fireplaces. These provide routes for access, but also if animals, mammals or birds are trapped and die, the carcasses attract insects. Birds’ nests are a further source of insects. Housekeeping is often stretched in the London properties, and there is greater staff turnover. There is clearly a range of characteristics of the properties that offers possible explanations for their high insect catches.
The catch rate for some individual species is shown in Figures 3c-3h, and here we see apparent differences in the regional distribution of insects (p < 0.001 for each figure, Chi-squared test DoF = 4). London for example shows a high frequency of silverfish, woolly bear larvae and webbing clothes moths compared with other territories. A high frequency of furniture beetles is notable in the Southeast and West, but to some extent these are driven by its prevalence in the Annexe and Casemate Tunnels (in the Southeast) and Atcham stores (under closure) in the West. In general the beetle is only found at 1.7% occupancy in relation to the number of traps throughout the record, although at just over 3% the Southeast and the West (p = 0.02, Mann–Whitney U test), but in the Tunnels and Atcham stores it is at 5.1% and 5.2% respectively (significant difference between these sites and the Southeast and the West territories; p = 0.04, Mann–Whitney U test). The wood weevils (Pentarthrum and Euophryum), show similar patterns to the furniture beetle, with highest catch rates in the southeast (0.053) and the west (0.028). Plaster beetles (Lathridiidae), as discussed below, are frequent in the Southeast.
Variation between properties
Catch varies a great deal between individual properties. Annexe and Casemate Tunnels in the Southeast show high average catch rate for booklice (15.1 and 11.8 per trap; significant difference between these sites and the entire data set: p < 0.001, Mann–Whitney U test), but few silverfish. It is possible that the silverfish do not like the alkaline conditions in the tunnels, which are cut into fine-grained soft, white Dover chalk. Silverfish are frequent at Eltham Palace in London, which can be quite damp (8.1 per trap; significantly different from the complete London data: p < 0.001, Mann–Whitney U test). The insect is trapped at almost five times the rate of any other of the key sites. Woodlice are especially abundant at Walmer Castle, but also found at high frequency in the Tunnels and Atcham stores. Woolly bear larvae are notably abundant in the London properties; more abundant in the seven houses than at any other of the key properties discussed in this paper. They are most frequent at Apsley House with 0.70 per trap.
A high relative abundance of plaster beetles is found in Annexe Tunnels (6.0 per trap) and at Casemate Tunnels (0.45 per trap), but are less frequent elsewhere (p < 0.001, Mann–Whitney U test). Plaster beetles feed on mould so as the Tunnels are damp, the environment may be suitable for the growth of this food source. As mentioned before the furniture beetle (Anobium punctatum) is frequent in Atcham Stores, especially at Atcham Store No. 31 (0.13 per trap) and the Tunnels of the Southeast Territory. Although found in five of the key London properties, the furniture beetle is not caught at high frequency in the capital.
The descriptions outlined in the paragraph above suggest such great variability on a property-by-property basis that it becomes hard to draw clear conclusions. There seems little relationship between the patterns of species catch observed in the properties. Of course some insects such as booklice are the most prevalent insect at almost every site, but the catch rate varies enormously.
London properties are more constant in terms of the number of traps laid over the years and the nature of the properties, so seemed possible to test whether the distributions of trapped insects show different statistics. The low catch of many insects restricted the chi-square test to a contingency table of the most abundant insects: woolly bears, booklice, silverfish and woodlice. The test suggested that counts of these insects from the seven London properties do not follow a consistent distribution (p = 0.001, Chi-squared test, DoF = 18). This lends support to the individualistic nature of the properties with respect to the insects trapped.
Seasonal variation of catch
Statistics of infestation
where I k is the number of traps with k insects, T is the number of traps and λ is the average number of insects caught per trap.
An analysis of the frequency distribution of the catch of woolly bears is displayed as triangles in Figure 7a. Although their abundance is very much less, with only some 4000 caught in total, reflecting an average of 0.19 larvae per trap, the number of multiple catches on the same trap is much higher than expected (shown as a dotted line) on the basis of a Poisson distribution.
This non-random distribution also repeated at the level of the individual property where unexpectedly high numbers of insects are to be found in individual traps. Figure 7b shows woolly bear catch at Ranger’s House, Apsley House and Down House. Again the distribution does not follow that expected on the basis of a Poisson distribution (the dotted line shows the expectation for Ranger’s House, but those for the other properties are similar). Once again there are too many traps with no insects, and too many that show infestations. This non-Poisson nature of the distribution reflects the potential for infestation. The drivers of infestation are not entirely clear, but for example recent work of Dalin et al.  has shown that infestations spread more rapidly in monoculture environments. Historic interiors might also represent are rather undifferentiated environment for insects.
Statistics of species combinations
The number of listed species found on individual traps from all territories
The probability of one species being found with another can be easily estimated as the product of the two probabilities;
p12 = p1 p2
p12 = p1p2
In Figure 8a we can see that booklice and silverfish [i], booklice and woodlice [ii], booklice and woolly bears [iii], silverfish and woolly bears [iv] and booklice and plaster beetles [v] were found together more often than expected. Silverfish and woodlice [vi] and woolly bears and woodlice [vii] (Figure 8b) lie close to the diagonal line and are thus near to the expected frequency. Although in smaller numbers, brown house moths (Hofmannophila pseudospretella) are found at higher than expected association with booklice [viii], and not surprisingly woolly bears are strongly associated with the varied carpet beetle [ix]- an adult form of the woolly bear. There is a lower than expected association between plaster beetles and silverfish [x] (Figure 8b).
Pheromone and plastic floor traps
Several hundred traps were laid that included pheromones as attractants for either the webbing clothes moth or less commonly the case bearing clothes moth. The largest number were placed in the key London properties after 2004. The catch rate was 2.67 moths per trap where pheromone was present, as expected e.g.  much greater than the 0.065 moths per trap caught when it was absent; a more than thirty-fold increase in catch rate (p < 0.001, Mann–Whitney U test). Some 29% of the traps showed the presence of moths in the case of pheromone traps compared with 3.3% presence in the absence of the pheromone. This situation was repeated in the Southeast where the catch rate was 3.18 and 0.03 for the pheromone and non-pheromone traps (p < 0.001, Mann–Whitney U test). Fewer pheromone traps were laid for the case bearing clothes moth and most of these were in the West. However, here the situation was much as expected with increases in the catch rate 0.97 with pheromones and 0.05 without (p < 0.001, Mann–Whitney U test) and the occupancy (respectively 20% and 2.1%).
There is a possibility that plastic floor traps (PFTs) designed to exclude bats or dust and debris, may also have lowered insect catch. The largest number (83) of these were used at Kenilworth Castle where the overall catch rate was lower (p < 0.07, Mann–Whitney U test) in PFTs, 0.65 compared with 2.49 for the 276 simple blunder traps. Taking a single species, the crustacean woodlouse showed a catch rate in the PFTs of 0.385, compared to 0.511 for the blunder traps (p < 0.04, Mann–Whitney U test). The catch in PFTs tends to be dominated by crawling insects and larger flying insects may be excluded, so these traps may be less than ideal  and may fail to give a representative distribution of all insect species. Even on the open blunder traps not all insects are caught with the same efficiency, so it is necessary to be cautious about the extent to which the insects trapped represent the abundance in properties [13, 14].
Certain insects such as booklice or silverfish are the most frequent in many properties. Not surprisingly the tunnels in the Southeast showed a high capture of booklice along with a relatively high frequency of plaster beetles. London properties were also notable in the frequency in which insects were trapped. Silverfish (Lepisma saccharina) had a very high catch rate at Eltham Palace. Woolly bear larvae were most frequent at Apsley House, but generally abundant on traps in all the London properties. The individualistic nature of insects present in properties was clear. London properties have high visitor flow and exchange of collections, are more difficult to maintain, and housekeeping is often stretched, which may be part of the explanation for the abundance of insects found there.
Trends over time were difficult to establish. The total rate of catch of booklice (Liposcelis bostrychophila) increased over time, but when we examined the seven houses in London, where there were rather similar approaches to sampling, there was no evidence of similar trends or correlations. Determining the relationship between changes in catch and climate or environmental factors requires more thorough analysis. The distribution of insects in traps did not follow the Poisson distribution, expected if insects were randomly distributed. This is evident from the occasional capture of large numbers of insects and hints at the importance of infestation. Some insects showed a tendency to be associated with other insects at a higher frequency than expected, but there were no especially obvious entomological reasons for this, apart from the occasional association of adults with their larvae. Webbing and case bearing clothes moths were more abundant in pheromone traps, confirming effective capture through the use of attractants. Traps that are covered to prevent dust or admission of bats may not give a true indication of the distribution of other insect species.
The trapping reported here provides clues for the management of pests in heritage properties. However, the individual character of pests at a given property means that the approach needs to be tailored to the needs of the property. It also suggests that although an insect might occur in low numbers in some periods, the possibility of infestation remains. The statistical analysis undertaken here did not identify any obvious drivers of high insect abundance in the traps. Future work will need to examine the trends in catch more carefully and ascertain the importance of environmental factors and food availability.
This work forms part of the English Heritage contract NHPP 2C2.301-6282. It would not have been possible without the efforts of staff who set the traps and recorded the results, sometimes for more than a decade. We would like to thank Helen Lloyd and other anonymous commentators for many useful observations on the first version of the MS, and to the referee who drew attention to Anthrenus spp. and Attagenus spp. being particularly attracted by dead insects.
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